IL-12 (IL12A & IL12B Heterodimer) Recombinant Protein | IL-12 recombinant protein

Human IL-12 Protein (IL12A & IL12B Heterodimer) Protein (His Tag)

Cat. Num. AAA2545172

• Gene Names: IL12B; CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
• Purity: > 95 % as determined by SDS-PAGE.
• Synonyms: IL-12 (IL12A & IL12B Heterodimer); Human IL-12 Protein (IL12A & IL12B Heterodimer) Protein (His Tag); IL12A & IL12B; IL-12 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 328

• Application Notes: The recombinant human consists 529 amino acids and predicts a molecular mass of 59.6 kDa.
• Predicted N Terminal: Ile 23
• Endotoxin: < 1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL-12 recombinant protein

A DNA sequence encoding the human IL12 complex composed of IL12B (NP_002178.2) (Met1-Ser328) and the mature form of human IL12A (NP_000873.2) (Arg 23 - Ser 219) linked by a polypeptide linker was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 24497438
• NCBI GeneID: 3593
• NCBI Accession #: NP_002178.2
• NCBI GenBank Nucleotide #: NM_002187.2
• UniProt Accession #: P29460
• Molecular Weight: 37,169 Da
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12B
• NCBI Official Symbol: IL12B
• NCBI Official Synonym Symbols: CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
• NCBI Protein Information: interleukin-12 subunit beta
• UniProt Protein Name: Interleukin-12 subunit beta
• UniProt Gene Name: IL12B
• UniProt Synonym Gene Names: NKSF2; IL-12B; CLMF p40; NKSF2

NCBI Description

This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]

Uniprot Description

IL12B: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: extracellular region; extracellular space; interleukin-12 complex

Molecular Function: cytokine activity; growth factor activity; identical protein binding; interleukin-12 alpha subunit binding; interleukin-12 receptor binding; interleukin-23 receptor binding; protein binding; protein heterodimerization activity

Biological Process: cell cycle arrest; cell migration; defense response to Gram-negative bacterium; interferon-gamma biosynthetic process; natural killer cell activation; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of smooth muscle cell proliferation; positive regulation of activated T cell proliferation; positive regulation of cell adhesion; positive regulation of defense response to virus by host; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-17 production; positive regulation of lymphocyte proliferation; positive regulation of memory T cell differentiation; positive regulation of mononuclear cell proliferation; positive regulation of natural killer cell activation; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of natural killer cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of NK T cell activation; positive regulation of NK T cell proliferation; positive regulation of osteoclast differentiation; positive regulation of T cell mediated cytotoxicity; positive regulation of T cell proliferation; positive regulation of T-helper 1 type immune response; positive regulation of tissue remodeling; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of cytokine biosynthetic process; regulation of tyrosine phosphorylation of Stat1 protein; response to UV-B; sexual reproduction; T-helper 1 type immune response; T-helper cell differentiation

Disease: Immunodeficiency 29