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IL-12 recombinant protein

Recombinant Rat IL-12 Protein

Applications
ELISA, Western Blot, SDS-Page
Purity
>95% as determined by reducing SDS-PAGE
Synonyms
IL-12; Recombinant Rat IL-12 Protein; NKSF1; NKSF2; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; IL-12 recombinant protein
Ordering
For Research Use Only!
Host
Human Cells
Purity/Purification
>95% as determined by reducing SDS-PAGE
Form/Format
Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
Sequence
MWELEKDVYV VEVDWRPDAP GETVTLTCDS PEEDDITWTS DQRRGVIGSG KTLTITVREF LDAGQYTCHR GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VHRNTDLKFN IKSSSSSPES RAVTCGRASL SAEKVTLNQR DYEKYSVACQ EDVTCPTAEE TLPIELVVEA QQQNKYENYS TSFFIRDIIK PDPPKNLQVK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKTK ETEEECNQKG AFLVEKTSAE VQCKGANICV QAQDRYYNSS CSKWTCVPCR GRSHHHHHH& RVIPVSGPAK CLNQSQNLLK TTDDMVRTAR EKLKHYSCTA GDIDHEDITR DKTSTLEACL PLELHKNESC LATKETSSII RGSCLPPQKT SLMMTLCLGS IYEDLKMYQS EFQAINAALQ SHNHQQITLD RNMLMAIDEL MRSLNHSGET LHQKAPMGEA DPYRVKMKLC ILLHAFSTRV MTINRVMNYL SSSHHHHHH
Sequence Length
335
Applicable Applications for IL-12 recombinant protein
ELISA (EIA), Western Blot (WB), SDS-PAGE
Endotoxin
Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for IL-12 recombinant protein
Recombinant Rat IL-12 Protein is produced by mammalian expression system and the target gene encoding Met23-Ser335 (p40) & Arg23-Ser215 (p35) is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Interleukin-12 p40
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
Il12b
UniProt Entry Name
Q9R278_RAT

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

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Product Notes

The IL-12 il12b (Catalog #AAA8305373) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL-12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), SDS-PAGE. Researchers should empirically determine the suitability of the IL-12 il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MWELEKDVYV VEVDWRPDAP GETVTLTCDS PEEDDITWTS DQRRGVIGSG KTLTITVREF LDAGQYTCHR GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VHRNTDLKFN IKSSSSSPES RAVTCGRASL SAEKVTLNQR DYEKYSVACQ EDVTCPTAEE TLPIELVVEA QQQNKYENYS TSFFIRDIIK PDPPKNLQVK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKTK ETEEECNQKG AFLVEKTSAE VQCKGANICV QAQDRYYNSS CSKWTCVPCR GRSHHHHHH& RVIPVSGPAK CLNQSQNLLK TTDDMVRTAR EKLKHYSCTA GDIDHEDITR DKTSTLEACL PLELHKNESC LATKETSSII RGSCLPPQKT SLMMTLCLGS IYEDLKMYQS EFQAINAALQ SHNHQQITLD RNMLMAIDEL MRSLNHSGET LHQKAPMGEA DPYRVKMKLC ILLHAFSTRV MTINRVMNYL SSSHHHHHH. It is sometimes possible for the material contained within the vial of "IL-12, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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