IL-12 Recombinant Protein | IL12 recombinant protein

Mouse IL-12 Recombinant

Cat. Num. AAA553281

• Purity: >90%, as determined by SDS-PAGE
• Synonyms: IL-12; Mouse IL-12 Recombinant; CLMF ; il-12; il12; IL12 recombinant protein

• Host: HEK Cells
• Purity/Purification: >90%, as determined by SDS-PAGE
• Form/Format: Recombinant mouse Interleukin-12 was lyophilized from a 0.2 um filtered PBS solution pH 7.4.
• Sequence: MCQSRYLLFL ATLALLNHLS LARVIPVSGP ARCLSQSRNL LKTTDDMVKT AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST RVVTINRVMG YLSSA MCPQKLTISW FAIVLLVSPL MAMWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS
• Sequence Length: 236

• Domain: Extracellular
• Host Note: DNA sequence encoding extracellular domain of mouse IL-12A fused to a C-terminal polyHis tag and a DNA sequence encoding extracellular domain of mouse IL-12B fused to a C-terminal polyHis tag were co-expressed in HEK cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Recombinant mouse Interleukin-12 is a heterodimer consisting of 528 amino acid residue subunits (203 subunit A + 324 subunit B), and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL12 recombinant protein

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBM

Product Categories/Family for IL12 recombinant protein

Receptors CD

References

• expression, engineering and characterization of the tumor-targeting heterodimeric immunocytokine f8-IL12 .Protein Eng. Des. Sel., Aug 2010; 23: 653 - 661.
• il-12 p35, IL12/23p40 and il-23 p19 subunit expression in giant-cell arteritis (gca) .Ann Rheum Dis, Jun 2007; 66: 140.
• treatment of experimental murine pancreatic peritoneal carcinomatosis with fibroblasts genetically modified to express IL12: a role for peritoneal innate immunity .Gut, Jan 2007; 56: 107 - 114.
• prophylaxis against carcinogenesis in three kinds of unestablished tumor models via IL12-gene-engineered mscs .Carcinogenesis, Dec 2006; 27: 2434 - 2441.
• combination of local, non-viral IL12 gene therapy and systemic paclitaxel chemotherapy in a syngeneic id8 mouse model for human ovarian cancer .Anticancer Res, Sep 2006; 26: 3223 - 3228.