IL-10 receptor beta (IL10RB) Recombinant Protein | IL10RB recombinant protein
Mouse IL-10 receptor beta (IL10RB) Recombinant
Purity
>90%, as determined by SDS-PAGE and HPLC
Synonyms
IL-10 receptor beta (IL10RB); Mouse IL-10 receptor beta (IL10RB) Recombinant; Mouse IL-10 receptor beta Recombinant; CSIF ; il-10; il10; il-10rb; il10rb; cd210; IL10RB recombinant protein
Host
HEK Cells
Purity/Purification
>90%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant IL10RB was lyophilized from a 0.2 um filtered PBS solution pH 7.4.
Sequence
MAPCVAGWLG GFLLVPALGM IPPPEKVRMN SVNFKNILQW EVPAFPKTNL TFTAQYESYR SFQDHCKRTA STQCDFSHLS KYGDYTVRVR AELADEHSEW VNVTFCPVED TIIGPPEMQI ESLAESLHLR FSAPQIENEP ETWTLKNIYD SWAYRVQYWK NGTNEKFQVV SPYDSEVLRN LEPWTTYCIQ VQGFLLDQNR TGEWSEPICE RTGNDEITPS WIVAIILIVS VLVVFLFLLG CFVVLWLIYK KTKHTFRSGT SLPQHLKEFL GHPHHSTFLL FSFPPPEEAE VFDKLSIISE ESEGSKQSPE DNCASEPPSD PGPRELESKD EAPSPPHDDP KLLTSTSEV
Sequence Length
349
Domain
Extracellular
Host Note
DNA sequence encoding extracellular domain of Mouse IL-10RB fused to a C-terminal polyHis tag was expressed in HEK cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant Mouse Interleukin-10RB is a monomer consisting of 212 amino acid residue subunits, and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for IL10RB recombinant protein
IL-10 is produced by mouse Th2-cells following their stimulation by lectins. The main source for B-cell derived IL10 in mice are Ly1 B-cells that express CD5 (Ly1) and CD11.In humans IL10 is produced by activated CD8 (+) peripheral blood T-cells, by T-helper CD4 (+) T-cell clones after both antigen-specific and polyclonal activation, by B- cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells.IL10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin10 shows 73 % amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL2 and TNF-beta in Th1 T-helper subpopulations of T-cells but not of Th2 T-helper cells. This activity is antagonized by IL4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of Th1 and Th2 cells.
Product Categories/Family for IL10RB recombinant protein
References
• genetic variation in the il-10 pathway modulates severity of acute graft-versus-host disease following hematopoietic cell transplantation: synergism between il-10 genotype of patient and IL-10 receptor genotype of donor .Blood, Dec 2005; 106: 3995 - 4001.
• association of il-10 and IL-10 receptor gene polymorphisms and graft-versus-host disease following hematopoietic cell transplantation. .Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 421.
• the expanded family of class ii cytokines that share the IL-10 receptor-2 (il-10r2) chain .J. Leukoc. Biol., Aug 2004; 76: 314 - 321.
• cutting edge: il-26 signals through a novel receptor complex composed of il-20 receptor 1 and IL-10 receptor 2 .J. Immunol., Feb 2004; 172: 2006 - 2010.
• inhibition of IL-10 receptor function in alveolar macrophages by toll-like receptor agonists .J. Immunol., Feb 2004; 172: 2613 - 2620.
• association of il-10 and IL-10 receptor gene polymorphisms and graft-versus-host disease following hematopoietic cell transplantation. .Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 421.
• the expanded family of class ii cytokines that share the IL-10 receptor-2 (il-10r2) chain .J. Leukoc. Biol., Aug 2004; 76: 314 - 321.
• cutting edge: il-26 signals through a novel receptor complex composed of il-20 receptor 1 and IL-10 receptor 2 .J. Immunol., Feb 2004; 172: 2006 - 2010.
• inhibition of IL-10 receptor function in alveolar macrophages by toll-like receptor agonists .J. Immunol., Feb 2004; 172: 2613 - 2620.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
39,774 Da
NCBI Official Full Name
Interleukin-10 receptor subunit beta
UniProt Protein Name
Interleukin-10 receptor subunit beta
Protein Family
UniProt Gene Name
Il10rb
UniProt Synonym Gene Names
Crfb4; IL-10 receptor subunit beta; IL-10R subunit beta; IL-10RB; CRF2-4; IL-10R subunit 2; IL-10R2
UniProt Entry Name
I10R2_MOUSE
Uniprot Description
IL10RB: Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29. Defects in IL10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Belongs to the type II cytokine receptor family.
Protein type: Membrane protein, integral
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: protein binding; interleukin-10 receptor activity
Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus
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Product Notes
The IL10RB il10rb (Catalog #AAA553243) is a Recombinant Protein produced from HEK Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MAPCVAGWLG GFLLVPALGM IPPPEKVRMN SVNFKNILQW EVPAFPKTNL TFTAQYESYR SFQDHCKRTA STQCDFSHLS KYGDYTVRVR AELADEHSEW VNVTFCPVED TIIGPPEMQI ESLAESLHLR FSAPQIENEP ETWTLKNIYD SWAYRVQYWK NGTNEKFQVV SPYDSEVLRN LEPWTTYCIQ VQGFLLDQNR TGEWSEPICE RTGNDEITPS WIVAIILIVS VLVVFLFLLG CFVVLWLIYK KTKHTFRSGT SLPQHLKEFL GHPHHSTFLL FSFPPPEEAE VFDKLSIISE ESEGSKQSPE DNCASEPPSD PGPRELESKD EAPSPPHDDP KLLTSTSEV. It is sometimes possible for the material contained within the vial of "IL-10 receptor beta (IL10RB), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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