IL10RA recombinant protein

IL10RA, 22-235aa, Human, His tag, Baculovirus

Cat. Num. AAA205890

• Gene Names: IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: IL10RA; 22-235aa; Human; His tag; Baculovirus; Interleukin-10 receptor subunit alpha; CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R; IL10RA recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.2mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSY DLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILG KIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS LLTSGEVGEF CVQVKPSVAS RSNKGMWSKE ECISLTRQYF TVTNHHHHHH
• Sequence Length: 578

• Applicable Applications for IL10RA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IL10RA recombinant protein

IL10RA, also known as Interleukin-10 receptor subunit alpha, is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. It has been shown to interact with Interleukin 10 and Janus kinase 1. Recombinant human IL10RA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL10RA recombinant protein

Hormones & Cytokines; Immunology

References

Josephson K, et al. (2001) Acta Crystallogr D Biol Crystallogr. 57 (Pt 12):1908-11.; Usacheva A, et al. (2002) J Immunol. 169 (3):1302-8.;

NCBI and Uniprot Product Information

• NCBI GI #: 222136575
• NCBI GeneID: 3587
• NCBI Accession #: NP_001549
• NCBI GenBank Nucleotide #: NM_001558.3
• UniProt Accession #: Q13651; A8K6I0; B0YJ27
• Molecular Weight: 25.2kDa (220aa) 228-40kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: interleukin-10 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 10 receptor subunit alpha
• NCBI Official Symbol: IL10RA
• NCBI Official Synonym Symbols: CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
• NCBI Protein Information: interleukin-10 receptor subunit alpha
• UniProt Protein Name: Interleukin-10 receptor subunit alpha
• Protein Family: Interleukin-10 receptor
• UniProt Gene Name: IL10RA
• UniProt Synonym Gene Names: IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1

NCBI Description

The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

Receptor for IL10; binds IL10 with a high affinity.