Interleukin-9 ELISA Kit | IL-9 elisa kit

Interleukin-9 (IL-9)

Cat. Num. AAA590065

• Gene Names: IL9; P40; HP40; IL-9
• Synonyms: Interleukin-9; Interleukin-9 (IL-9); IL-9 elisa kit

• Specificity: This sandwich ELISA can detect both natural and recombinant human IL-9. This kit exhibits no significant cross-reactivity with human IL-1alpha, IL-1beta, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, G-CSF, GM-CSF, TGF-beta1, TNF-alpha, TNF-beta, MCAF, MCP-3, bFGF, EGF, IFN-gamma.
• Sequence Length: 144

• Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of human IL-9 was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human IL-9 calculated from calibrate I diluted standard curve was <0.343pg/mL.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of eight in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 8 different assays.
• Intended Uses: This Human Interleukin 9 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 9 (IL-9) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-9 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-9 present is bound by the immobilized antibody. After washing away unbound substances, a biotin-linked antibody specific for IL- 9 is added to the wells. Following a wash to remove unbound biotin labelled antibody, an avidin-HRP conjugate is added to each wells. Avidin binds to biotin labelled antibody with high affinity. After TMB substrate solution is added to the wells, colour will develop in proportion to the amount of IL-9 bound in the initial step. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-9 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-9 concentration (pg/mL). The concentration of IL-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: Interleukin 9(IL-9) was initially discovered as a 40KD glycoprotein with T-cell growth factor activity in mice (1). Human IL-9 gene was identified based on its remarkable homogeneity with the gene of mouse IL-9 (2) and was mapped to chromosome 5. Human IL-9 has been demonstrated to be a stimulator for hematopoiesis and be associated with human malignant lymphoma (3, 4, 5, 6). In addition, IL-9 and IL-9 receptor expression was found to be implicated with autoimmune and allergic diseases (7, 8). IL-9 has been studied as a therapeutic target for asthma (7, 9) because of its implication with the disease. IL-9 can be produced by Th2 cells under the stimulation of TGF-beta and IL-4 and has been shown to play a role in the differentiation of Th17 cells and Treg function (10, 11). In recent years, IL-9 has received renewed attention because a unique type of innate lymphoid cells has been identified as the main cell type that expresses IL-9 in vivo and the IL-9 secreting cells present in skin appear to be mediate inhibition of melanoma growth in mice (12,13,14,15). The same IL-9 secreting cells have been identified in human and the cells are currently evaluated as a new tumour treatment strategy.

Product Categories/Family for IL-9 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 10834980
• NCBI GeneID: 3578
• NCBI Accession #: NP_000581.1
• NCBI GenBank Nucleotide #: NM_000590.1
• Molecular Weight: 15,909 Da
• NCBI Official Full Name: interleukin-9
• NCBI Official Synonym Full Names: interleukin 9
• NCBI Official Symbol: IL9
• NCBI Official Synonym Symbols: P40; HP40; IL-9
• NCBI Protein Information: interleukin-9; T-cell growth factor p40; cytokine P40; homolog of mouse T cell and mast cell growth factor 40; p40 T-cell and mast cell growth factor; p40 cytokine
• UniProt Protein Name: Interleukin-9
• Protein Family: Interleukin
• UniProt Gene Name: IL9
• UniProt Entry Name: IL9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]

Uniprot Description

IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; interleukin-9 receptor binding; cytokine activity

Biological Process: positive regulation of cell proliferation; immune response; inflammatory response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process