Rabbit Interleukin 8 ELISA Kit | IL-8 elisa kit

Rabbit Interleukin 8 ELISA Kit

Cat. Num. AAA763266

• Gene Names: CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• Reactivity: Rabbit
• Synonyms: Interleukin 8; Rabbit Interleukin 8 ELISA Kit; IL-8 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL-8. No significant cross-reactivity or interference between IL-8 and analogues was observed.
• Sequence Length: 99

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10834978
• NCBI GeneID: 3576
• NCBI Accession #: NP_000575.1
• NCBI GenBank Nucleotide #: NM_000584.3
• Molecular Weight: 11,098 Da
• NCBI Official Full Name: interleukin-8
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 8
• NCBI Official Symbol: CXCL8
• NCBI Official Synonym Symbols: IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
• NCBI Protein Information: interleukin-8
• UniProt Protein Name: Interleukin-8
• UniProt Gene Name: CXCL8
• UniProt Synonym Gene Names: IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF
• UniProt Entry Name: IL8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]

Uniprot Description

IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 4q13-q21

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: chemokine activity; interleukin-8 receptor binding; protein binding

Biological Process: angiogenesis; calcium-mediated signaling; cell cycle arrest; cell motility; cellular protein metabolic process; chemotaxis; embryonic gut development; G-protein coupled receptor protein signaling pathway; immune response; induction of positive chemotaxis; inflammatory response; negative regulation of cell proliferation; negative regulation of G-protein coupled receptor protein signaling pathway; neutrophil activation; neutrophil chemotaxis; positive regulation of angiogenesis; receptor internalization; regulation of cell adhesion; regulation of retroviral genome replication; response to molecule of bacterial origin; signal transduction; unfolded protein response