Human Interleukin 2 (IL-2) ELISA Kit | IL-2 elisa kit

Human Interleukin 2 (IL-2) ELISA kit

Cat. Num. AAA260418

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Reactivity: Human
• Synonyms: Interleukin 2 (IL-2); Human Interleukin 2 (IL-2) ELISA kit; IL-2 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 153

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/mL-15.6 pg/mL
• Sensitivity: Up to 5 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL-2 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human IL-2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 28178861
• NCBI GeneID: 3558
• NCBI Accession #: NP_000577.2
• NCBI GenBank Nucleotide #: NM_000586.3
• Molecular Weight: 17,628 Da
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2; aldesleukin; T cell growth factor; involved in regulation of T-cell clonal expansion
• UniProt Protein Name: Interleukin-2
• UniProt Gene Name: IL2
• UniProt Synonym Gene Names: IL-2; TCGF
• UniProt Entry Name: IL2_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Oncoprotein; Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; interleukin-2 receptor binding; kinase activator activity; cytokine activity; glycosphingolipid binding; carbohydrate binding; kappa-type opioid receptor binding

Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; protein kinase C activation; positive regulation of cell proliferation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of B cell apoptosis; positive regulation of inflammatory response; negative regulation of apoptosis