Related Product Information for IL-16 elisa kit
Principle of the assay: IL-16 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-16. Standards or samples are then added to the microtiter plate wells and IL-16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-16 are added to each well to "sandwich" the IL-16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-16 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-16 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-16 elisa kit
NCBI Description
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Uniprot Description
IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 15q26.3
Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus
Molecular Function: cytokine activity
Biological Process: transcription, DNA-dependent; viral reproduction; regulation of transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis
Product Notes
The Rat IL-16 il16 (Catalog #
AAA721637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA721637 ELISA Kit recognizes Rat IL-16.
It is sometimes possible for the material contained within the vial of
"Interleukin 16, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
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aspect of this datasheet at
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