Goat Interleukin 16 ELISA Kit | IL-16 elisa kit

Goat Interleukin 16 ELISA Kit

Cat. Num. AAA737135

• Gene Names: IL16; LCF; NIL16; PRIL16; prIL-16
• Reactivity: Goat
• Synonyms: Interleukin 16; Goat Interleukin 16 ELISA Kit; IL-16 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This IL-16 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat IL-16. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-16 elisa kit

Principle of the Assay: IL-16 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-16 antibody and an IL-16-HRP conjugate. The assay sample and buffer are incubated together with IL-16-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-16 concentration since IL-16 from samples and IL-16-HRP conjugate compete for the anti-IL-16 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-16 from the sample, fewer sites are left to bind IL-16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-16 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL-16 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 116283877
• NCBI GeneID: 3603
• Molecular Weight: 66,646 Da
• NCBI Official Full Name: IL16 protein, partial
• NCBI Official Synonym Full Names: interleukin 16
• NCBI Official Symbol: IL16
• NCBI Official Synonym Symbols: LCF; NIL16; PRIL16; prIL-16
• NCBI Protein Information: pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor
• UniProt Protein Name: Pro-interleukin-16
• UniProt Gene Name: IL16
• UniProt Synonym Gene Names: IL-16; LCF
• UniProt Entry Name: IL16_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: transcription, DNA-dependent; viral reproduction; regulation of transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis