Porcine Inhibitor of KB Kinase Beta ELISA Kit | IKKbeta elisa kit

Porcine Inhibitor of KB Kinase Beta ELISA Kit

Cat. Num. AAA739794

• Gene Names: Ikbkb; IKK2; IKK-2; IKK[b]; IKKbeta; AI132552; IKK-beta
• Reactivity: Porcine
• Synonyms: Inhibitor of KB Kinase Beta; Porcine Inhibitor of KB Kinase Beta ELISA Kit; IKKbeta elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This IKKbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IKKbeta. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IKKbeta elisa kit

Principle of the Assay: IKKbeta ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IKKbeta. Standards or samples are then added to the microtiter plate wells and IKKbeta if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IKKbeta present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for IKKbeta are added to each well to "sandwich" the IKKbeta immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IKKbeta and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IKKbeta concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IKKbeta elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 229576825
• NCBI GeneID: 16150
• NCBI Accession #: NP_001153246.1
• NCBI GenBank Nucleotide #: NM_001159774.1
• Molecular Weight: 86,690 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 1
• NCBI Official Synonym Full Names: inhibitor of kappaB kinase beta
• NCBI Official Symbol: Ikbkb
• NCBI Official Synonym Symbols: IKK2; IKK-2; IKK[b]; IKKbeta; AI132552; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; NFKBIKB; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: Ikbkb
• UniProt Synonym Gene Names: Ikkb; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_MOUSE

Uniprot Description

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: Kinase, protein; EC 2.7.11.10; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Cellular Component: internal side of plasma membrane; intracellular membrane-bound organelle; membrane; cytoplasm; IkappaB kinase complex; nucleus; cytosol

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: serine phosphorylation of STAT protein; B cell homeostasis; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; phosphorylation; protein amino acid phosphorylation; neurite development; activation of NF-kappaB transcription factor