Western Blot (WB)
(Western Blot (WB) analysis of TWIK-1 (V322) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)
Rabbit TWIK-1 Polyclonal Antibody | anti-TWIK-1 antibody
TWIK-1 (V322) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
TWIK-1; Polyclonal Antibody; TWIK-1 (V322) Polyclonal Antibody; Potassium channel subfamily K member 1; Inward rectifying potassium channel protein TWIK-1; Potassium channel KCNO1; KCNK1; HOHO1; KCNO1; TWIK1; anti-TWIK-1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TWIK-1 (V322) polyclonal antibody detects endogenous levels of TWIK-1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
336
Applicable Applications for anti-TWIK-1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 301-350 of Human TWIK-1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of TWIK-1 (V322) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of TWIK-1 (V322) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)
Related Product Information for anti-TWIK-1 antibody
K+ channels are divided into three subclasses, reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions, and include TASK, TREK, TRAAK and TWIK. TWIK-1 mRNA is expressed abundantly in brain and at lower levels in lung, kidney and skeletal muscle. The molecular weight of TWIK-1 in mouse brain is 40 kDa under reducing conditions. TWIK-2 shares low sequence homology with other mammalian family group members, and only 34% homology with TWIK-1. Human TWIK-2 is expressed in pancreas, placenta and heart, while mouse TWIK-2 is expressed in liver. TWIK-2 is inhibited by intracellular, but not extracellular, acidification.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 38kDa
NCBI Official Full Name
potassium channel subfamily K member 1
UniProt Protein Name
Potassium channel subfamily K member 1
UniProt Gene Name
KCNK1
UniProt Synonym Gene Names
HOHO1; KCNO1; TWIK1
UniProt Entry Name
KCNK1_HUMAN
Uniprot Description
KCNK1: Weakly inward rectifying potassium channel. Homodimer (Potential). Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1q42-q43
Cellular Component: voltage-gated potassium channel complex; brush border membrane; apical plasma membrane; plasma membrane; endosome
Molecular Function: inward rectifier potassium channel activity
Biological Process: response to nicotine; synaptic transmission; potassium ion transport
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Product Notes
The TWIK-1 kcnk1 (Catalog #AAA3003682) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TWIK-1 (V322) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TWIK-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the TWIK-1 kcnk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TWIK-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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