IkappaBalpha Phospho-Regulation Antibody Sampler Kit | IkBaK antibody sampler kit
IkappaBalpha Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunoprecipitation
Synonyms
IkappaBalpha Phospho-Regulation; IkBaK antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for IkBaK antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for IkBaK antibody sampler kit
Kit Summary: The IkappaBalpha phospho-regulation antibody sampler kit can be used to detect IkappaBalpha phosphorylation on Ser-32/Ser-36, Tyr-42, and Tyr-305. The kit also includes a polyclonal antibody to monitor total IkappaBalpha expression levels and secondary reagentsfor detection of these antibodies.
Background: The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkappaBproteins. Activation of IkappaBalpha occurs through both serine and tyrosine phosphorylation events. Activation throughphosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release andnuclear translocation of active NF-kappaB. This pathway of IkappaBalpha regulation occurs in response to various NF-kappaB-activatingagents, such as TNFalpha, interleukins, LPS, and irradiation. An alternative pathway for IkappaBalpha regulation occurs throughtyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growthfactors. This phosphorylation can lead to degradation of IkappaBalpha and NF-kappaB-activation. In contrast, Tyr-305 phosphorylationby c-Abl has been implicated in IkappaBalpha nuclear translocation and inhibition of NF-kappaB-activation. Thus, tyrosinephosphorylation of IkappaBalpha may be an important regulatory mechanism in NF-kappaB signaling.
Background: The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkappaBproteins. Activation of IkappaBalpha occurs through both serine and tyrosine phosphorylation events. Activation throughphosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release andnuclear translocation of active NF-kappaB. This pathway of IkappaBalpha regulation occurs in response to various NF-kappaB-activatingagents, such as TNFalpha, interleukins, LPS, and irradiation. An alternative pathway for IkappaBalpha regulation occurs throughtyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growthfactors. This phosphorylation can lead to degradation of IkappaBalpha and NF-kappaB-activation. In contrast, Tyr-305 phosphorylationby c-Abl has been implicated in IkappaBalpha nuclear translocation and inhibition of NF-kappaB-activation. Thus, tyrosinephosphorylation of IkappaBalpha may be an important regulatory mechanism in NF-kappaB signaling.
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Product Notes
The Human, Rat, Mouse IkBaK (Catalog #AAA474385) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474385 Antibody Sampler Kit recognizes Human, Rat, Mouse IkBaK. AAA Biotech's IkappaBalpha Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the IkBaK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IkappaBalpha Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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