Testing Data
(Chemical structure of Sodium Citrate )
Citrate buffer IHC Related Reagent
Citrate buffer
Applications
Immunohistochemistry
Synonyms
Citrate buffer; Citrate buffer ihc related reagent
Form/Format
3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
Powder
Powder
Applicable Applications for Citrate buffer ihc related reagent
IHC-P, HIER, PEFF tissues.
Application Notes
Used for the protocol of antigen retrieval in Immunohistochemistry (IHC)-paraffin embedded sections.
Composition
Sodium citrate H2O
pH
6
Volume of final solution
2000 mL
CAS
68-04-2
Molecular Formula
C6H5Na3O7
Chemical Sources
CheMBL, ChEMBL 1355, ChemSpider:5989, PubChem: 6224
Usage and Handling
See the IHC protocol for more specific usage instructions.
Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation.
Advice: In order to achieve sufficinet retrieval or avoid tissue destruction or over digestion, appropiate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody or tissue type after preliminary experimentation by the researcher.
Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation.
Advice: In order to achieve sufficinet retrieval or avoid tissue destruction or over digestion, appropiate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody or tissue type after preliminary experimentation by the researcher.
Preparation and Storage
Store at room temperature (RT) for one year.
Testing Data
(Chemical structure of Sodium Citrate )
Testing Data
(Chemical structure of Sodium Citrate )
Related Product Information for Citrate buffer ihc related reagent
Citrate buffer solution is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation.
Product Categories/Family for Citrate buffer ihc related reagent
Similar Products
Product Notes
The Citrate buffer (Catalog #AAA176570) is an IHC Related Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Citrate buffer can be used in a range of immunoassay formats including, but not limited to, IHC-P, HIER, PEFF tissues. Used for the protocol of antigen retrieval in Immunohistochemistry (IHC)-paraffin embedded sections. Researchers should empirically determine the suitability of the Citrate buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Citrate buffer, IHC Related Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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