IGSF8 recombinant protein

Recombinant Human IGSF8 Protein

Cat. Num. AAA8249216

• Gene Names: IGSF8; EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: IGSF8; Recombinant Human IGSF8 Protein; CD81P3; EWI2; KCT4; Immunoglobulin superfamily member 8; IgSF8; CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; EWI-2; Keratinocytes-associated transmembrane protein 4; KCT-4; LIR-D1; Prostaglandin regulatory-like protein; PGRL; CD316; IGSF8 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: REVLVPEGPL YRVAGTAVSI SCNVTGYEGP AQQNFEWFLY RPEAPDTALG IVSTKDTQFS YAVFKSRVVA GEVQVQRLQG DAVVLKIARL QAQDAGIYEC HTPSTDTRYL GSYSGKVELR VLPDVLQVSA APPGPRGRQA PTSPPRMTVH EGQELALGCL ARTSTQKHTH LAVSFGRSVP EAPVGRSTLQ EVVGIRSDLA VEAGAPYAER LAAGELRLGK EGTDRYRMVV GGAQAGDAGT YHCTAAEWIQ DPDGSWAQIA EKRAVLAHVD VQTLSSQLAV TVGPGERRIG PGEPLELLCN VSGALPPAGR HAAYSVGWEM APAGAPGPGR LVAQLDTEGV GSLGPGYEGR HIAMEKVASR TYRLRLEAAR PGDAGTYRCL AKAYVRGSGT RLREAASARS RPLPVHVREE GVVLEAVAWL AGGTVYRGET ASLLCNISVR GGPPGLRLAA SWWVERPEDG ELSSVPAQLV GGVGQDGVAE LGVRPGGGPV SVELVGPRSH RLRLHSLGPE DEGVYHCAPS AWVQHADYSW YQAGSARSGP VTVYPYMHAL DTVDHHHHHH
• Sequence Length: 613

• Applicable Applications for IGSF8 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for IGSF8 recombinant protein

Recombinant Human IGSF8 Protein is produced by our mammalian expression system and the target gene encoding Arg28-Thr579 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 331284207
• NCBI GeneID: 93185
• NCBI Accession #: NP_001193594.1
• NCBI GenBank Nucleotide #: NM_001206665.2
• UniProt Accession #: Q969P0; Q8NG09; Q96DP4; Q9BTG9
• Molecular Weight: 55,808 Da
• NCBI Official Full Name: immunoglobulin superfamily member 8
• NCBI Official Synonym Full Names: immunoglobulin superfamily member 8
• NCBI Official Symbol: IGSF8
• NCBI Official Synonym Symbols: EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• NCBI Protein Information: immunoglobulin superfamily member 8
• UniProt Protein Name: Immunoglobulin superfamily member 8
• Protein Family: Immunoglobulin superfamily
• UniProt Gene Name: IGSF8
• UniProt Synonym Gene Names: CD81P3; EWI2; KCT4; IgSF8; EWI-2; KCT-4; PGRL
• UniProt Entry Name: IGSF8_HUMAN

NCBI Description

This gene encodes a member the EWI subfamily of the immunoglobulin protein superfamily. Members of this family contain a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a variable number of immunoglobulin domains. This protein interacts with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. The encoded protein may also function as a tumor suppressor by inhibiting the proliferation of certain cancers. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

IGSF8: May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin- dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: integral to membrane; membrane; plasma membrane

Molecular Function: protein binding