Rabbit anti-Human PLAG1 Polyclonal Antibody | anti-PLAG1 antibody

PLAG1, NT (PLAG1, Zinc finger protein PLAG1, Pleiomorphic adenoma gene 1 protein) (Azide free) (HRP)

Cat. Num. AAA6334623

• Gene Names: PLAG1; PSA; SGPA; ZNF912
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLAG1; Polyclonal Antibody; NT (PLAG1; Zinc finger protein PLAG1; Pleiomorphic adenoma gene 1 protein) (Azide free) (HRP); Pleiomorphic adenoma gene 1 protein; anti-PLAG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PLAG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PLAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human PLAG1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PLAG1 Antibody (N-term) western blot analysis in NCI-H460,K562 cell line lysates (35ug/lane).This demonstrates the PLAG1 antibody detected the PLAG1 protein (arrow).)

Related Product Information for anti-PLAG1 antibody

Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-PLAG1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Patz, M., et al. Leuk. Lymphoma 51(8):1379-1381(2010). Declercq, J., et al. Diabetes 59(8):1957-1965(2010). Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010). Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010). Zhao, J., et al. BMC Med. Genet. 11, 96 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 167857796
• NCBI GeneID: 5324
• NCBI Accession #: NP_001108106.1; NP_002646.2
• NCBI GenBank Nucleotide #: NM_001114634.1
• UniProt Accession #: Q6DJT9; Q59GH8; Q9Y4L2; B4DLC2
• Molecular Weight: 46,474 Da
• NCBI Official Full Name: zinc finger protein PLAG1 isoform a
• NCBI Official Synonym Full Names: PLAG1 zinc finger
• NCBI Official Symbol: PLAG1
• NCBI Official Synonym Symbols: PSA; SGPA; ZNF912
• NCBI Protein Information: zinc finger protein PLAG1
• UniProt Protein Name: Zinc finger protein PLAG1
• Protein Family: Zinc finger protein
• UniProt Gene Name: PLAG1

NCBI Description

Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PLAG1: Transcription factor whose activation results in up- regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia. A chromosomal aberration involving PLAG1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with constituvely expressed beta-catenin/CTNNB1. Fusion occurs in the 5'-regulatory regions, leading to promoter swapping between the 2 genes and activation of PLAG1 expression in adenomas. The chimeric transcript is formed by fusion of CTNNB1 exon 1 to PLAG1 exon 3. Reciprocal fusion transcript consisting of PLAG1 exon 1 and CTNNB1 exon 2-16 is also revealed in some adenomas. Translocation t(3;8)(p21;q12) with transcription elongation factor SII/TCEA1. The fusion transcript is composed of 5'-non-coding sequences as well as 63 nucleotides of the coding region of TCEA1 fused to the acceptor splice site of PLAG1 exon 3. The fusion transcript encodes a truncated TCEA1-PLAG1 protein of 90 AA as well as an apparently normal PLAG1 protein. Reciprocal fusion transcript PLAG1-TCEA1 is also present in one adenoma. Translocation t(5;8)(p13;q12) with leukemia inhibitory factor receptor LIFR. This fusion occured in the 5'-non-coding sequences of both genes, exchanging regulatory control element while preserving the coding sequences. Translocation t(6;8)(p21.3- 22;q13) with Coiled-coil-helix-coiled-coil-helix domain-containing protein 7/CHCHD7. Fusion occurs in the 5' regulatory regions, leading to promoter swapping and up-regulation of PLAG1 expression. Ectopic expression of PLAG1 under the control of promoters of distinct translocation partner genes is a general pathogenetic mechanism for pleiomorphic adenomas with 8q aberrations. These fusion genes are likely to be found in adenomas with normal karyotype as this subgroup of tumors also exhibit PLAG1 activation. A chromosomal aberration involving PLAG1 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with hyaluronic acid synthase 2/HAS2 results in promoter swapping and activation of PLAG1 expression. The breakpoint of HAS2 gene is in PLAG1 intron 1, whereas its coding sequence starts at exon 2 or exon 3. Translocation t(7;8)(p22;q13) with collagen 1A2/COL1A2. Fusion transcript COL1A2-PLAG1 as well as HAS2-PLAG1 encode a full-length PLAG1 protein. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 8q12.1

Cellular Component: nuclear speck; nucleus

Molecular Function: DNA binding transcription factor activity; metal ion binding

Biological Process: apoptosis; gland morphogenesis; multicellular organism growth; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Salivary Gland Adenoma, Pleomorphic