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SDS-Page

IGSF8 recombinant protein

IGSF8, 28-579aa, Human, His-tag, Baculovirus

Gene Names
IGSF8; EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
IGSF8; 28-579aa; Human; His-tag; Baculovirus; Immunoglobulin superfamily member 8; CD316; CD81P3; EWI-2; EWI2; KCT-4; LIR-D1; PGRL; IGSF8 recombinant protein
Ordering
For Research Use Only!
Host
Baculovirus
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 30% glycerol.
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
ADLREVLVPE GPLYRVAGTA VSISCNVTGY EGPAQQNFEW FLYRPEAPDT ALGIVSTKDT QFSYAVFKSR VVAGEVQVQR LQGDAVVLKI ARLQAQDAGI YECHTPSTDT RYLGSYSGKV ELRVLPDVLQ VSAAPPGPRG RQAPTSPPRM TVHEGQELAL GCLARTSTQK HTHLAVSFGR SVPEAPVGRS TLQEVVGIRS DLAVEAGAPY AERLAAGELR LGKEGTDRYR MVVGGAQAGD AGTYHCTAAE WIQDPDGSWA QIAEKRAVLA HVDVQTLSSQ LAVTVGPGER RIGPGEPLEL LCNVSGALPP AGRHAAYSVG WEMAPAGAPG PGRLVAQLDT EGVGSLGPGY EGRHIAMEKV ASRTYRLRLE AARPGDAGTY RCLAKAYVRG SGTRLREAAS ARSRPLPVHV REEGVVLEAV AWLAGGTVYR GETASLLCNI SVRGGPPGLR LAASWWVERP EDGELSSVPA QLVGGVGQDG VAELGVRPGG GPVSVELVGP RSHRLRLHSL GPEDEGVYHC APSAWVQHAD YSWYQAGSAR SGPVTVYPYM HALDTHHHHH H
Sequence Length
613
Applicable Applications for IGSF8 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for IGSF8 recombinant protein
IGSF8, also known as Immunoglobulin superfamily member 8, is a member of the immunoglobulin protein superfamily. It interact with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. Also, this protein may function as a tumor suppressor by inhibiting the proliferation of certain cancers. Recombinant human IGSF8 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for IGSF8 recombinant protein
References
Kolesnikova TV., et al, (2004) Blood. 103:3013-3019.; Wang HX., et al, (2015) Cell Res. 25:370-385.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59.6kDa (561aa) 50-70KDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
immunoglobulin superfamily member 8
NCBI Official Synonym Full Names
immunoglobulin superfamily member 8
NCBI Official Symbol
IGSF8
NCBI Official Synonym Symbols
EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
NCBI Protein Information
immunoglobulin superfamily member 8
UniProt Protein Name
Immunoglobulin superfamily member 8
UniProt Gene Name
IGSF8
UniProt Synonym Gene Names
CD81P3; EWI2; KCT4; IgSF8; EWI-2; KCT-4; PGRL

NCBI Description

This gene encodes a member the EWI subfamily of the immunoglobulin protein superfamily. Members of this family contain a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a variable number of immunoglobulin domains. This protein interacts with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. The encoded protein may also function as a tumor suppressor by inhibiting the proliferation of certain cancers. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.

Research Articles on IGSF8

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Product Notes

The IGSF8 igsf8 (Catalog #AAA206679) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's IGSF8 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the IGSF8 igsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADLREVLVPE GPLYRVAGTA VSISCNVTGY EGPAQQNFEW FLYRPEAPDT ALGIVSTKDT QFSYAVFKSR VVAGEVQVQR LQGDAVVLKI ARLQAQDAGI YECHTPSTDT RYLGSYSGKV ELRVLPDVLQ VSAAPPGPRG RQAPTSPPRM TVHEGQELAL GCLARTSTQK HTHLAVSFGR SVPEAPVGRS TLQEVVGIRS DLAVEAGAPY AERLAAGELR LGKEGTDRYR MVVGGAQAGD AGTYHCTAAE WIQDPDGSWA QIAEKRAVLA HVDVQTLSSQ LAVTVGPGER RIGPGEPLEL LCNVSGALPP AGRHAAYSVG WEMAPAGAPG PGRLVAQLDT EGVGSLGPGY EGRHIAMEKV ASRTYRLRLE AARPGDAGTY RCLAKAYVRG SGTRLREAAS ARSRPLPVHV REEGVVLEAV AWLAGGTVYR GETASLLCNI SVRGGPPGLR LAASWWVERP EDGELSSVPA QLVGGVGQDG VAELGVRPGG GPVSVELVGP RSHRLRLHSL GPEDEGVYHC APSAWVQHAD YSWYQAGSAR SGPVTVYPYM HALDTHHHHH H. It is sometimes possible for the material contained within the vial of "IGSF8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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