Monkey Immunoglobulin G1 ELISA Kit | IgG1 elisa kit
Monkey Immunoglobulin G1 ELISA Kit
Reactivity
Monkey
Synonyms
Immunoglobulin G1; Monkey Immunoglobulin G1 ELISA Kit; IgG1 elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of IgG1. No significant cross-reactivity or interference between IgG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IgG1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ug/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IgG1 elisa kit
Intended Uses: This IgG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IgG1. This ELISA kit for research use only!
Principle of the Assay: IgG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IgG1 antibody and an IgG1-HRP conjugate. The assay sample and buffer are incubated together with IgG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgG1 concentration since IgG1 from samples and IgG1-HRP conjugate compete for the anti-IgG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IgG1 from the sample, fewer sites are left to bind IgG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IgG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IgG1 antibody and an IgG1-HRP conjugate. The assay sample and buffer are incubated together with IgG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgG1 concentration since IgG1 from samples and IgG1-HRP conjugate compete for the anti-IgG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IgG1 from the sample, fewer sites are left to bind IgG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IgG1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
immunoglobulin G1 Fc region, partial
