IGFBP-7 recombinant protein

RECOMBINANT HUMAN IGFBP-7

Cat. Num. AAA232420

• Gene Names: IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• Applications: ELISA, Western Blot
• Purity: >98% by SDS PAGE and HPLC analysis.
• Synonyms: IGFBP-7; RECOMBINANT HUMAN IGFBP-7; IGFBP-7 recombinant protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis.
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Approximate Protein Concentration: 0.1 mg/ml after reconstitution (varies by lot)
• Sequence Length: 279

• Applicable Applications for IGFBP-7 recombinant protein: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This recombinant protein may be as a positive control in western blotting and may be run under either reducing or non-reducing conditions.; ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 0.25 ml distilled water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage the additiona of 0.1% BSA is recommended.; Preparation
• Buffer Solution: 10mM Sodium Phosphate, pH6.8
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for IGFBP-7 recombinant protein

IGFBP-7 (insulin-like growth factor binding protein-7) is a secreted protein which belongs to a family of IGF-BPs which regulate the biological activities and distribution of the insulin-like growth factors (IGFs), through binding to IGF-I and IGF-II. IGFBP-7 is expressed in many tumour types and acts as a suppressor of breast and prostate cancer cell lines. IGFBP-7 inhibits IGF-induced differentiation of skeletal muscle (myogenesis) and has implications in bone formation.

NCBI and Uniprot Product Information

• NCBI GI #: 359465608
• NCBI GeneID: 3490
• NCBI Accession #: NP_001240764.1
• NCBI GenBank Nucleotide #: NM_001253835.1
• UniProt Accession #: Q16270; Q07822; Q53YE6; Q9UCA8; B4E1N2; B7Z9W7
• Molecular Weight: 26.4kD (257 amino acid residues).
• NCBI Official Full Name: insulin-like growth factor-binding protein 7 isoform 2
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 7
• NCBI Official Symbol: IGFBP7
• NCBI Official Synonym Symbols: AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• NCBI Protein Information: insulin-like growth factor-binding protein 7; IGF-binding protein 7; IGFBP-rP1; PGI2-stimulating factor; angiomodulin; prostacyclin-stimulating factor; tumor-derived adhesion factor
• UniProt Protein Name: Insulin-like growth factor-binding protein 7
• UniProt Gene Name: IGFBP7
• UniProt Synonym Gene Names: MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF
• UniProt Entry Name: IBP7_HUMAN

NCBI Description

This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]

Uniprot Description

IGFBP7: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Defects in IGFBP7 are the cause of retinal arterial macroaneurysm with supravalvular pulmonic stenosis (RAMSVPS). RAMSVPS is an autosomal recessive condition characterized by the bilateral appearance of 'beading' along the major retinal arterial trunks, with the subsequent formation of macroaneurysms. Affected individuals also have supravalvular pulmonic stenosis, often requiring surgical correction.

Protein type: Cell adhesion; Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; protein binding

Biological Process: negative regulation of cell proliferation; response to cortisol stimulus; response to retinoic acid; response to heat; regulation of steroid biosynthetic process; regulation of cell growth; cell adhesion; inner ear development; embryo implantation; cellular response to hormone stimulus

Disease: Retinal Arterial Macroaneurysm With Supravalvular Pulmonic Stenosis