Testing Data
Mouse Immunoglobulin A ELISA Kit | IgA elisa kit
Mouse Immunoglobulin A ELISA Kit
Reactivity
Mouse
Synonyms
Immunoglobulin A; Mouse Immunoglobulin A ELISA Kit; IgA elisa kit
Reactivity
Mouse
Form/Format
Complete kit
Preparation and Storage
Store at 4 degree C
Expiration: 12 months from manufacture
Expiration: 12 months from manufacture
Testing Data
Testing Data
Related Product Information for IgA elisa kit
Immunoglobulin A (IgA) is the main immunoglobulin in mucous secretions and the second most abundant immunoglobulin in serum. It defends against inhaled and ingested pathogens by binding an Fc receptor on myeloid leukocytes. The equivalent mouse receptor has not yet been identified. Human IgA has two distinct subclasses and is monomeric in serum while mouse IgA does not have subclasses and is dimeric in serum. The sensitive quantitative measurement of total mouse IgA antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluid samples is easily performed with this 96 well strip format ELISA kit.The kit does not cross react significantly with rat, pig, sheep, dog, rabbit, cyno monkey, rhesus monkey, or human IgA. The concentration of IgA was found to be 0.4 mg/ml in NIH Swiss mouse serum and 0.7 mg/ml in BALB/C mouse serum. The assay measures mouse IgA in the 0.1-100 ng/ml range. Samples giving mouse IgA levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:200,000 dilution for serum is suggested for best results. Mouse IgA will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-mouse IgA primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of mouse IgA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse IgA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
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Product Notes
The Mouse IgA (Catalog #AAA136066) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA136066 ELISA Kit recognizes Mouse IgA. It is sometimes possible for the material contained within the vial of "Immunoglobulin A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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