Interferon-gamma Recombinant Protein | IFN-gamma recombinant protein

Human Interferon-gamma Recombinant

Cat. Num. AAA553052

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: Interferon-gamma; Human Interferon-gamma Recombinant; ifn-g; ifng; gamma interferon; IFN-gamma recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interferon gamma was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.2.
• Sequence: MKYTSYILAF QLCIVLGSLG CYCQDPYVKE AENLKKYFNA GHSDVADNGT LFLGILKNWK EESDRKIMQS QIVSFYFKLF KNFKDDQSIQ KSVETIKEDM NVKFFNSNKK KRDDFEKLTN YSVTDLNVQR KAIHELIQVM AELSPAAKTG KRKRSQMLFR GRRASQ
• Sequence Length: 166

• Domain: 4_helix_cytokine_core
• Host Note: Optimized DNA sequence encoding Human Interferon gamma mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native Interferon gamma is generated by the proteolytic removal of the signal peptide, the molecule has a calculated molecular mass of approximately 17 kDa. Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 144 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IFN-gamma recombinant protein

IFN-gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFN gamma receptors are expressed on all types of human cells with the exception of mature erythrocytes.IFN-gamma- receptor complexes are rapidly internalized by endocytosis. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. Interferon-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. The growth inhibitory activities of IFN-gamma are more pronounced than those of the other interferons. However, the main biological activity of IFN-gamma appears to be immunomodulatory in contrast to the other interferons that are mainly antiviral. n T-helper cells IL2 induces the synthesis of IFN-gamma and other cytokines. IFN- gamma acts synergistically with IL1 and IL2 and appears to be required for the expression of IL2 receptors on the cell surface of T- lymphocytes. Blocking of the IL2 receptor by specific antibodies also inhibits the synthesis of IFN-gamma.

Product Categories/Family for IFN-gamma recombinant protein

Cytokines

References

• diminished frequency of nk cells and cd4+IFN-g+ t cells in patients with mycotic corneal ulcers .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1945.
• IFN-g-stimulated mesenchymal stromal cells acquire the ability to cross-present exogenous soluble antigens and lead to an effective immune response. .Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 4898.
• regulation of tlr expression and activation in human mesenchymal stromal cells by ifn-a, IFN-g and tnf-a. .Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 1790.
• positive association of tnf-857 and ifn g 874 gene polymorphisms with chronic fatigue syndrome .Ann Rheum Dis, Jun 2005; 64: 344.
• measurement of functional cytomegalovirus (cmv) -specific t cells by interferon-gamma-enzyme-linked immunospot (IFN-g elispot) assay specific for a single immunodominant epitope enables the sensitive evaluation of immune recovery for cmv in patients after allogeneic stem cell transplantation (hsct) with a reduced-intensity regimen (rist). .Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 3157.