IFNA4 recombinant protein

IFNA4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8118607

• Gene Names: IFNA4; INFA4; IFN-alpha4a
• Purity: >88 % as determined by SDS-PAGE
• Synonyms: IFNA4; IFNA4 Protein; Human; Recombinant (His Tag); Human IFNalpha4/IFNa4/Interferon alpha-4 Protein (His Tag); interferon; alpha 4; IFN-alpha4a Protein; INFA4 Protein; MGC142200 Protein; IFNA4 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >88 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 176aa
• Sequence: Met1-Asp189

• Species: Human
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys24
• Protein Construction: |A DNA sequence encoding the human IFNalpha 4a (NP_066546.1)) (Met 1-Asp 189) was fused with a polyhistidine tag at the C-terminus
• Activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV). The ED50 for this effect is typically 2-8 pg/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV). The ED50 for this effect is typically 2-8 pg/mL.)

Related Product Information for IFNA4 recombinant protein

Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

References

Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.

NCBI and Uniprot Product Information

• NCBI GI #: 10835103
• NCBI GeneID: 3441
• NCBI Accession #: NP_066546.1
• NCBI GenBank Nucleotide #: NM_021068.2
• Molecular Weight: 21,808 Da
• NCBI Official Full Name: interferon alpha-4
• NCBI Official Synonym Full Names: interferon, alpha 4
• NCBI Official Symbol: IFNA4
• NCBI Official Synonym Symbols: INFA4; IFN-alpha4a
• NCBI Protein Information: interferon alpha-4; IFN-alpha-4; interferon alpha-4B; interferon alpha-76; interferon alpha-M1