Chicken Interferon gamma ELISA Kit | IFN-gamma elisa kit
Chicken Interferon gamma (IFN-gamma) ELISA Kit
Reactivity
Chicken
Synonyms
Interferon gamma; Chicken Interferon gamma (IFN-gamma) ELISA Kit; IFN-gamma elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of Chicken IFN-?. No significant cross-reactivity or interference between Chicken IFN-? and analogues was observed.
Sequence Length
166
Samples
serum, plasma and other biological fluids
Detection Range
31.25 pg/mL - 2000 pg/mL
Sensitivity
10.2 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for IFN-gamma elisa kit
Intended Uses: For the quantitative detection of Chicken Interferon ? (IFN-?) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IFN-? in Chicken serum, plasma. An antibody specific for IFN-? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-? is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-? bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IFN-? in Chicken serum, plasma. An antibody specific for IFN-? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-? is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-? bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,953 Da
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
IFNG
NCBI Protein Information
interferon gamma
UniProt Protein Name
Interferon gamma
Protein Family
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
Uniprot Description
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons ().
Research Articles on IFN-gamma
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Product Notes
The Chicken IFN-gamma ifng (Catalog #AAA280703) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280703 ELISA Kit recognizes Chicken IFN-gamma. It is sometimes possible for the material contained within the vial of "Interferon gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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