Typical Testing Data/Standard Curve (for reference only)
Mouse Interferon beta ELISA Kit | IFN-beta elisa kit
Mouse Interferon beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IFNbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IFNbeta antibody and an IFNbeta-HRP conjugate. The assay sample and buffer are incubated together with IFNbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNbeta concentration since IFNbeta from samples and IFNbeta-HRP conjugate compete for the anti-IFNbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNbeta from the sample, fewer sites are left to bind IFNbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNbeta concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9p21
Cellular Component: extracellular space; extracellular region
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; defense response to bacterium; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response
Research Articles on IFN-beta
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Product Notes
The Mouse IFN-beta ifnb1 (Catalog #AAA723426) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723426 ELISA Kit recognizes Mouse IFN-beta. It is sometimes possible for the material contained within the vial of "Interferon beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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