Pig Interferon alpha ELISA Kit | IFN-alpha elisa kit
Pig Interferon alpha (IFN-alpha) ELISA Kit
Reactivity
Pig
Synonyms
Interferon alpha; Pig Interferon alpha (IFN-alpha) ELISA Kit; IFN-alpha elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig IFN-alpha. No significant cross-reactivity or interference between Pig IFN-alpha and analogues was observed.
Sequence Length
195
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15 pg/mL - 600 pg/mL
Sensitivity
10 pg/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for IFN-alpha elisa kit
Intended Uses: For the quantitative detection of Pig Interferon alpha (IFN-alpha) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody preparation specific for IFN-alpha and incubated. Then substrate solution A and B are added to each well. Only those wells that contain IFN-alpha, HRP-Conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IFN-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This assay employs a two-site sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFN-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody preparation specific for IFN-alpha and incubated. Then substrate solution A and B are added to each well. Only those wells that contain IFN-alpha, HRP-Conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IFN-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,319 Da
NCBI Official Full Name
interferon-alpha
NCBI Official Synonym Full Names
interferon, omega 1
NCBI Official Symbol
IFNW1
NCBI Protein Information
interferon omega-1; interferon alpha-II-1; IFN-omega 1, interferon omega-1
UniProt Protein Name
Interferon omega-1
Protein Family
UniProt Gene Name
IFNW1
UniProt Entry Name
IFNW1_HUMAN
Uniprot Description
IFNW1: Belongs to the alpha/beta interferon family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; cell cycle arrest; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein
Research Articles on IFN-alpha
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Product Notes
The Pig IFN-alpha ifnw1 (Catalog #AAA280420) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280420 ELISA Kit recognizes Pig IFN-alpha. It is sometimes possible for the material contained within the vial of "Interferon alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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