Typical Testing Data/Standard Curve (for reference only)
Mouse IFN-alpha (Interferon alpha) CLIA Kit | IFN-a clia kit
Mouse IFN-alpha (Interferon alpha) CLIA Kit
Reactivity
Mouse
Synonyms
IFN-alpha (Interferon alpha); Mouse IFN-alpha (Interferon alpha) CLIA Kit; Mouse IFN-a (Interferon a) CLIA Kit; Mouse IFN-a; IFN-a clia kit
Reactivity
Mouse
Specificity
This kit recognizes natural and recombinant IFN-alpha. No significant cross-reactivity or interference between IFN-alpha and analogues was observed.
Sequence Length
195
Assay Type
Sandwich
Detection Range
7.813-500pg/mL
Sensitivity
Min: 4.688pg/mL; Max: 500pg/mL
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IFN-a clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of IFN-alpha concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IFN-alpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IFN-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IFN-alpha. You can calculate the concentration of IFN-alpha in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IFN-alpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IFN-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IFN-alpha. You can calculate the concentration of IFN-alpha in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,319 Da
NCBI Official Full Name
interferon-alpha
NCBI Official Synonym Full Names
interferon, omega 1
NCBI Official Symbol
IFNW1
NCBI Protein Information
interferon omega-1
UniProt Protein Name
Interferon omega-1
UniProt Gene Name
IFNW1
UniProt Entry Name
IFNW1_HUMAN
Uniprot Description
IFNW1: Belongs to the alpha/beta interferon family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; response to virus; cytokine and chemokine mediated signaling pathway; natural killer cell activation during immune response; cell cycle arrest; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response
Research Articles on IFN-a
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Product Notes
The Mouse IFN-an ifnw1 (Catalog #AAA2530822) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530822 CLIA Kit recognizes Mouse IFN-a. It is sometimes possible for the material contained within the vial of "IFN-alpha (Interferon alpha), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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