Interferon-induced helicase C domain-containing protein 1 (Ifih1) Recombinant Protein | Ifih1 recombinant protein

Recombinant Mouse Interferon-induced helicase C domain-containing protein 1 (Ifih1) , partial

Cat. Num. AAA1472275

• Gene Names: Ifih1; Hlcd; MDA5; RLR-2; Helicard; 9130009C22Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Interferon-induced helicase C domain-containing protein 1 (Ifih1); Recombinant Mouse Interferon-induced helicase C domain-containing protein 1 (Ifih1); partial; Ifih1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 700-1025. Partial
• Sequence: KLIKLRNTILEQFTRSEESSRGIIFTKTRQSTYALSQWIMENAKFAEVGVKAHHLIGAGHSSEVKPMTQTEQKEVISKFRTGEINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVTSSGSGVTEREIVNDFREKMMYKAINRVQNMKPEEYAHKILELQVQSILEKKMKVKRSIAKQYNDNPSLITLLCKNCSMLVCSGENIHVIEKMHHVNMTPEFKGLYIVRENKALQKKFADYQTNGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVNFKNNSPKKQYKKWVELPIRFPDLDYSEYCLYSDED

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Ifih1 recombinant protein

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 257096038
• NCBI GeneID: 71586
• NCBI Accession #: NP_001157949.1
• NCBI GenBank Nucleotide #: NM_001164477.1
• UniProt Accession #: Q8R5F7; Q3U6S2; Q68EM4; Q8BYC9; Q8BZ01; Q8K5C7; Q8R144; Q8VE79; Q99KS4; Q9D2Z5; A2AUY7
• Molecular Weight: 110,842 Da
• NCBI Official Full Name: interferon-induced helicase C domain-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: interferon induced with helicase C domain 1
• NCBI Official Symbol: Ifih1
• NCBI Official Synonym Symbols: Hlcd; MDA5; RLR-2; Helicard; 9130009C22Rik
• NCBI Protein Information: interferon-induced helicase C domain-containing protein 1
• UniProt Protein Name: Interferon-induced helicase C domain-containing protein 1
• Protein Family: Interferon-induced helicase C domain-containing protein
• UniProt Gene Name: Ifih1
• UniProt Synonym Gene Names: Helicard; MDA-5; RLR-2

Uniprot Description

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.