IsocitRe dehydrogenase (IDH3) Blocking Peptide | IDH3A blocking peptide

IsocitRe dehydrogenase (IDH3) Antibody (C-term) Blocking peptide

• Synonyms: IsocitRe dehydrogenase (IDH3); IsocitRe dehydrogenase (IDH3) Antibody (C-term) Blocking peptide; Isocitrate dehydrogenase [NAD] subunit alpha; mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha; IDH3A; IDH3A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human Isocitrate dehydrogenase. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 366

• Cellular Location: Mitochondrion.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 1708399
• NCBI GeneID: 3419
• UniProt Accession #: P50213; Q9H3X0; D3DW83
• Molecular Weight: 31,381 Da
• NCBI Official Full Name: Isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 3 (NAD(+)) alpha
• NCBI Official Symbol: IDH3A
• NCBI Protein Information: isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH3A
• UniProt Entry Name: IDH3A_HUMAN

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]

Uniprot Description

IDH3A: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 1.1.1.41; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 15q25.1-q25.2

Cellular Component: mitochondrial matrix; mitochondrion; nucleus

Biological Process: tricarboxylic acid cycle