Western Blot (WB)
(Western blot analysis of Phospho-PFKM (Ser74) in lysates of HeLa?, using Phospho-PFKM (Ser74) Antibody.)
Rabbit PFKM Polyclonal Antibody | anti-PFKM antibody
Phospho-PFKM (Ser74) Antibody
Gene Names
PFKM; GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
PFKM; Polyclonal Antibody; Phospho-PFKM (Ser74) Antibody; 6 Phosphofructokinase Muscle Type; 6-phosphofructokinase; 6-phosphofructokinase muscle type; EC 2.7.1.1; EC 2.7.1.11; GSD7; K6PF_HUMAN; MGC8699; muscle type; PFK; PFK-A; PFKA; PFKL; PFKP; PFKX; Phosphofructo 1 Kinase Isozyme A; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphofructokinase M; Phosphofructokinase; muscle; polypeptide X; Phosphofructokinase-M; Phosphohexokinase; anti-PFKM antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-PFKM (Ser74) Antibody detects endogenous levels of PFKM only when phosphorylated at Ser74.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
780
Applicable Applications for anti-PFKM antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA (Peptide): 1:20000-1:40000
ELISA (Peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human PFKM around the phosphorylation site of Ser74.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Phospho-PFKM (Ser74) in lysates of HeLa?, using Phospho-PFKM (Ser74) Antibody.)
Western Blot (WB)
(Western blot analysis of Phospho-PFKM (Ser74) in lysates of HeLa?, using Phospho-PFKM (Ser74) Antibody.)
Related Product Information for anti-PFKM antibody
Description: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Post Translational Modifications: GlcNAcylation decreases enzyme activity.
Subunit Structure: Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable). Interacts (via C-terminus) with HK1 (via N-terminal spermatogenic cell-specific region) (By similarity).
Similarity: Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.
Post Translational Modifications: GlcNAcylation decreases enzyme activity.
Subunit Structure: Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable). Interacts (via C-terminus) with HK1 (via N-terminal spermatogenic cell-specific region) (By similarity).
Similarity: Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 85 kDa
Predicted: 86 kDa
Predicted: 86 kDa
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NCBI Official Synonym Full Names
phosphofructokinase, muscle
NCBI Official Symbol
PFKM
NCBI Official Synonym Symbols
GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, muscle type
UniProt Protein Name
6-phosphofructokinase, muscle type
Protein Family
UniProt Gene Name
PFKM
UniProt Synonym Gene Names
PFKX; PFK-A
UniProt Entry Name
K6PF_HUMAN
NCBI Description
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
Research Articles on PFKM
Similar Products
Product Notes
The PFKM pfkm (Catalog #AAA9610424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PFKM (Ser74) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's PFKM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the PFKM pfkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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