Isocitrate dehydrogenase Recombinant Protein | IDHC recombinant protein

Recombinant mouse Isocitrate dehydrogenase [NADP] cytoplasmic

Cat. Num. AAA9420333

• Gene Names: Idh1; Id-1; Idpc; Idh-1; AI314845; AI788952; E030024J03Rik
• Reactivity: Mouse
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Isocitrate dehydrogenase; Recombinant mouse Isocitrate dehydrogenase [NADP] cytoplasmic; Cytosolic NADP-isocitrate dehydrogenaseIDPNADP (+)-specific ICDHOxalosuccinate decarboxylase; IDHC recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial of the Full Length of 1-414aa, 7-407aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

NCBI and Uniprot Product Information

• NCBI GI #: 162417977
• NCBI GeneID: 15926
• NCBI Accession #: NP_001104790.1
• NCBI GenBank Nucleotide #: NM_001111320.1
• UniProt Accession #: O88844; Q3UAV7
• Molecular Weight: 49.2kD
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 1 (NADP+), soluble
• NCBI Official Symbol: Idh1
• NCBI Official Synonym Symbols: Id-1; Idpc; Idh-1; AI314845; AI788952; E030024J03Rik
• NCBI Protein Information: isocitrate dehydrogenase [NADP] cytoplasmic
• UniProt Protein Name: Isocitrate dehydrogenase [NADP] cytoplasmic
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: Idh1
• UniProt Synonym Gene Names: IDH

Uniprot Description

IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML).

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 1 C2|1 32.91 cM

Cellular Component: cytoplasm; cytosol; mitochondrion; peroxisome

Molecular Function: (R)-2-hydroxyglutarate dehydrogenase activity; cadherin binding; isocitrate dehydrogenase (NADP+) activity; isocitrate dehydrogenase activity; magnesium ion binding; NADP binding; protein homodimerization activity; receptor binding

Biological Process: 2-oxoglutarate metabolic process; glutathione metabolic process; isocitrate metabolic process; response to organic cyclic substance; response to oxidative stress; response to steroid hormone stimulus