Mouse Isocitrate dehydrogenase [NADP] cytoplasmic ELISA Kit | Idh1 elisa kit

Mouse Isocitrate dehydrogenase [NADP] cytoplasmic ELISA Kit

Cat. Num. AAA2886463

• Gene Names: Idh1; Id-1; Idpc; Idh-1; AI314845; AI788952; E030024J03Rik
• Reactivity: Mouse
• Synonyms: Isocitrate dehydrogenase [NADP] cytoplasmic; Mouse Isocitrate dehydrogenase [NADP] cytoplasmic ELISA Kit; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; Idh1; 1.1.1.42; Idh1 elisa kit

• Reactivity: Mouse
• Sequence Length: 414

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.398 ng/mL
• Intra-Assay CV: <=8.3%
• Inter-Assay CV: <=10.5%
• Recovery: 90%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 162417977
• NCBI GeneID: 15926
• NCBI Accession #: NP_001104790.1
• NCBI GenBank Nucleotide #: NM_001111320.1
• UniProt Accession #: O88844; Q3UAV7
• Molecular Weight: 46,674 Da
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 1 (NADP+), soluble
• NCBI Official Symbol: Idh1
• NCBI Official Synonym Symbols: Id-1; Idpc; Idh-1; AI314845; AI788952; E030024J03Rik
• NCBI Protein Information: isocitrate dehydrogenase [NADP] cytoplasmic
• UniProt Protein Name: Isocitrate dehydrogenase [NADP] cytoplasmic
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: Idh1
• UniProt Synonym Gene Names: IDH
• UniProt Entry Name: IDHC_MOUSE

Uniprot Description

IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML).

Protein type: EC 1.1.1.42; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; Other Amino Acids Metabolism - glutathione

Cellular Component: cytoplasm; cytosol; mitochondrion; peroxisome

Molecular Function: isocitrate dehydrogenase (NADP+) activity; isocitrate dehydrogenase activity; magnesium ion binding; metal ion binding; NAD binding; NADP binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; protein homodimerization activity; receptor binding

Biological Process: 2-oxoglutarate metabolic process; glutathione metabolic process; glyoxylate cycle; isocitrate metabolic process; response to organic cyclic substance; response to oxidative stress; response to steroid hormone stimulus; tricarboxylic acid cycle