Insulin Degrading Enzyme Recombinant Protein | IDE recombinant protein

Recombinant Insulin Degrading Enzyme (IDE)

Cat. Num. AAA2029718

• Gene Names: IDE; INSULYSIN
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Insulin Degrading Enzyme; Recombinant Insulin Degrading Enzyme (IDE); IDE recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Concentration: 7.2 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AHTKPLLP SQLVRYREVQ LPDRGWFVYQ QRNEVHNNCG IEIYYQTDMQ STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA NGIQGLRFII QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL SAECAKYWGE IISQQYNFDR DNTEVAYLKT LTKEDIIKFY KEMLAVDAPRRHKVSVHVLA REMDSCPVVG EFP
• Sequence Length: 1019

• Applicable Applications for IDE recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 29.7kDa
• Accurate Molecular Mass (KD): 30kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ala753~Pro973
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 184556
• NCBI GeneID: 3416
• Molecular Weight: 54,240 Da
• NCBI Official Full Name: insulin-degrading enzyme
• NCBI Official Synonym Full Names: insulin-degrading enzyme
• NCBI Official Symbol: IDE
• NCBI Official Synonym Symbols: INSULYSIN
• NCBI Protein Information: insulin-degrading enzyme
• UniProt Protein Name: Insulin-degrading enzyme
• Protein Family: IgM protease
• UniProt Gene Name: IDE
• UniProt Synonym Gene Names: Insulinase
• UniProt Entry Name: IDE_HUMAN

NCBI Description

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

Uniprot Description

IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.56; Nuclear receptor co-regulator; Cell surface; Protease

Chromosomal Location of Human Ortholog: 10q23-q25

Cellular Component: nucleoplasm; peroxisomal matrix; extracellular space; cell surface; mitochondrion; cytoplasm; plasma membrane; peroxisome; nucleus; cytosol

Molecular Function: insulin binding; ubiquitin binding; protein binding; protein homodimerization activity; zinc ion binding; metalloendopeptidase activity; beta-amyloid binding; ATPase activity; glycoprotein binding; peptide binding; beta-endorphin binding; ATP binding; receptor binding

Biological Process: negative regulation of proteolysis; positive regulation of protein oligomerization; proteolysis involved in cellular protein catabolic process; viral reproduction; protein heterooligomerization; beta-amyloid metabolic process; insulin receptor signaling pathway; hormone catabolic process; determination of adult life span; proteolysis; protein homooligomerization; protein homotetramerization