Inducible T-Cell Co Stimulator (ICOS) Recombinant Protein | ICOS recombinant protein

Recombinant Inducible T-Cell Co Stimulator (ICOS)

Cat. Num. AAA2009323

• Gene Names: Icos; Ailim
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Inducible T-Cell Co Stimulator (ICOS); Recombinant Inducible T-Cell Co Stimulator (ICOS); ICOS recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- QI SCNYPETVQQ LKMQLFKDRE VLCDLTKTKG SGNTVSIKNP MSCPYQLSNN SVSFFLDNAD SSQGSYFLCS LSIFDPPPFQ EKNLSGGYLL IYE
• Sequence Length: 200

• Applicable Applications for ICOS recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gln39~Glu133 (Accession # Q9R1T7) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12018286
• NCBI GeneID: 64545
• NCBI Accession #: NP_072132.1
• NCBI GenBank Nucleotide #: NM_022610.1
• UniProt Accession #: Q9R1T7; Q9WVR9
• Molecular Weight: 12.2kDa
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T-cell co-stimulator
• NCBI Official Symbol: Icos
• NCBI Official Synonym Symbols: Ailim
• NCBI Protein Information: inducible T-cell costimulator; activation-inducible lymphocyte immunomediatory molecule
• UniProt Protein Name: Inducible T-cell costimulator
• Protein Family: Inducible T-cell costimulator
• UniProt Gene Name: Icos
• UniProt Synonym Gene Names: Ailim
• UniProt Entry Name: ICOS_RAT

NCBI Description

activation-inducible lymphocyte immunomediatory molecule; involved in the immune response in CD45RA-positive B-cells [RGD, Feb 2006]

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: integral to plasma membrane; external side of plasma membrane

Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation