ORP150 Recombinant Protein | HYOU1 recombinant protein

ORP150 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120796

• Gene Names: HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
• Purity: >97% as determined by SDS-PAGE
• Synonyms: ORP150; ORP150 Protein; Human; Recombinant (His Tag); Human ORP150/HYOU1/HSP12A Protein (His Tag); GRP-170 Protein; Grp170 Protein; HSP12A Protein; ORP-150 Protein; hypoxia up-regulated 1; HYOU1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met695-Leu994

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 695
• Tag: C-His
• Protein Construction: A DNA sequence encoding the C-terminal segment of human HSP12A (NP_001124463.1)(Met 695-Leu 994) was expressed, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HYOU1 recombinant protein

Background: Hypoxia up-regulated protein 1, also known as 15kDa oxygen-regulated protein, 17kDa glucose-regulated protein, ORP-15, GRP-17, and HYOU1, is a member of the heat shock protein 7 family. Seven members from four different heat shock protein (HSP) families were identified including HYOU1 (ORP15), HSPC1 (HSP86), HSPA5 (Bip), HSPD1 (HSP6), and several isoforms of the two testis-specific HSP7 chaperones HSPA2 and HSPA1L. HYOU1 is highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. It is highly expressed in the liver and pancreas. HYOU1 is also expressed in macrophages within aortic atherosclerotic plaques and in breast cancers. HYOU1 has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. It may play a role as a molecular chaperone and participate in protein folding. Suppression of HYOU1 is associated with accelerated apoptosis. It is suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein is up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness.

References

Ikeda J., et al.,(1997), Cloning and expression of cDNA encoding the human 150kDa oxygen-regulated protein, ORP150. Biochem. Biophys. Res. Commun. 230:94-99.Kaneda S., et al., (2000), Production of three distinct mRNAs of 150kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions.J. Biochem. 128:529-538.Takeuchi S., (2006), Molecular cloning, sequence, function and structural basis of human heart 150kDa oxygen-regulated protein, an ER chaperone.Protein J. 25:517-528.

NCBI and Uniprot Product Information

• NCBI GI #: 195976805
• NCBI GeneID: 10525
• NCBI Accession #: NP_001124463.1
• NCBI GenBank Nucleotide #: NM_001130991.2
• UniProt Accession #: Q9Y4L1; Q2I204; Q53H25; A8C1Z0; B7Z909
• Molecular Weight: 62,757 Da
• NCBI Official Full Name: hypoxia up-regulated protein 1
• NCBI Official Synonym Full Names: hypoxia up-regulated 1
• NCBI Official Symbol: HYOU1
• NCBI Official Synonym Symbols: Grp170; HSP12A; ORP150; GRP-170; ORP-150
• NCBI Protein Information: hypoxia up-regulated protein 1
• UniProt Protein Name: Hypoxia up-regulated protein 1
• Protein Family: Hypoxia up-regulated protein
• UniProt Gene Name: HYOU1
• UniProt Synonym Gene Names: GRP170; ORP150; ORP-150; GRP-170
• UniProt Entry Name: HYOU1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

HYOU1: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 11q23.1-q23.3

Cellular Component: smooth endoplasmic reticulum; focal adhesion; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region

Molecular Function: chaperone binding; ATP binding

Biological Process: receptor-mediated endocytosis; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response