Ghrelin Steroid
Ghrelin, Human (Ser3 acylated)
Applications
ELISA, Gel Super Shift Assay
Purity
Highly PurifiedHighly purified
Synonyms
Ghrelin; Human (Ser3 acylated); Ghrelin steroid
Host
Human
Purity/Purification
Highly Purified
Highly purified
Highly purified
Form/Format
Supplied as a liquid in PBS, pH 7.2
Applicable Applications for Ghrelin steroid
ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA, dot blot or Western Blot as a positive control to block antibody activity.
Dilution: ELISA: 50-100ng/well
ELISA: 50-100ng/well
Dilution: ELISA: 50-100ng/well
ELISA: 50-100ng/well
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Ghrelin steroid
Human full length ghrelin, 1-28-aa and n-octanoylated at ser3, peptide was synthesized and purified by hplc.
Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance. GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin ('ghre' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor. In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Ghrelin is the first known example of a bioactive peptide modified by an acyl acid.
Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake. Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects. Ghrelin administration stimulates GH secretion but also causes weight gain by increasing food intake and reduction in fat utilization and anti-Ghrelin IgG administration suppressed feeding.
Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance. GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin ('ghre' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor. In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Ghrelin is the first known example of a bioactive peptide modified by an acyl acid.
Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake. Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects. Ghrelin administration stimulates GH secretion but also causes weight gain by increasing food intake and reduction in fat utilization and anti-Ghrelin IgG administration suppressed feeding.
Product Categories/Family for Ghrelin steroid
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Product Notes
The Ghrelin (Catalog #AAA638991) is a Steroid produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ghrelin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA, dot blot or Western Blot as a positive control to block antibody activity. Dilution: ELISA: 50-100ng/well ELISA: 50-100ng/well. Researchers should empirically determine the suitability of the Ghrelin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ghrelin, Steroid" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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