CKMM native protein

CKMM protein

Cat. Num. AAA537486

• Gene Names: CKM; CKMM; M-CK
• Applications: User optimized
• Purity: >95% by SDS-PAGE
• Synonyms: CKMM; CKMM protein; CK-MM protein; Creatine Kinase-MM Isoenzyme Protein; CKMM native protein

• Host: Human skeletal muscle
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. Purified preperation in 25% (v/v) ethylene glycol, 10mM Tris, pH 7.5, 1mM EDTA, 5mM β-Meracaptoethanol with no preservative.
• Concentration: Specific Activity: 1182 IU/mg protein; 7.12 mg/ml protein (modified Lowry); 7.12 IU/ml (Roche CK reagent at 37°C) (varies by lot)
• Sequence Length: 381

• Applicable Applications for CKMM native protein: User optimized
• Protein Type: Native
• Biological Significance: Creatine kinase (CK) is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). This CK enzyme reaction is reversible, such that also ATP can be generated from PCr and ADP.
• Grade: Highly Purified
• Species: Human
• Biohazard: Donor Serum was tested and found negative for HBsAg, anti-HIV-1&2 and anti-HCV by FDA approved methods.
• Preparation and Storage: Store at 4°C for short term storage.; Aliquot and store at -20°C for long term storage.; Avoid repeated freeze/thaw cycles.

Related Product Information for CKMM native protein

Purified native Human CKMM protein

Product Categories/Family for CKMM native protein

Cardiac Markers; Native Protein

NCBI and Uniprot Product Information

• NCBI GI #: 21536288
• NCBI GeneID: 1158
• NCBI Accession #: NP_001815.2
• NCBI GenBank Nucleotide #: NM_001824.4
• NCBI Official Full Name: creatine kinase M-type
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: CKM
• NCBI Official Synonym Symbols: CKMM; M-CK
• NCBI Protein Information: creatine kinase M-type
• UniProt Protein Name: Creatine kinase M-type
• UniProt Gene Name: CKM
• UniProt Synonym Gene Names: CKMM
• UniProt Entry Name: KCRM_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Uniprot Description

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; Kinase, other; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; phosphorylation; creatine metabolic process