Human Receptor Interacting Serine Threonine Kinase 3 (RIPK3) RTU ELISA Kit | RIPK3 rtu elisa kit

Human Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit

Cat. Num. AAA4502241

• Gene Names: RIPK3; RIP3
• Reactivity: Human
• Synonyms: Receptor Interacting Serine Threonine Kinase 3 (RIPK3); Human Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit; RIP3; RIP-like protein kinase 3; Receptor-interacting protein 3; RIPK3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 518

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 11.7pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RIPK3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RIPK3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RIPK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RIPK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RIPK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40254844
• NCBI GeneID: 11035
• NCBI Accession #: NP_006862.2
• NCBI GenBank Nucleotide #: NM_006871.3
• UniProt Accession #: Q9Y572; Q5J795; Q5J796; Q6P5Y1; B4DJL9; C4AM87
• Molecular Weight: 25,326 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: receptor interacting serine/threonine kinase 3
• NCBI Official Symbol: RIPK3
• NCBI Official Synonym Symbols: RIP3
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK3
• UniProt Synonym Gene Names: RIP3; RIP-3

NCBI Description

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.