Human Crk Like Protein (CRKL) RTU ELISA Kit | CRKL rtu elisa kit

Human Crk Like Protein (CRKL) ELISA Kit

Cat. Num. AAA458576

• Reactivity: Human
• Synonyms: Crk Like Protein (CRKL); Human Crk Like Protein (CRKL) ELISA Kit; V-Crk Sarcoma Virus CT10 Oncogene Homolog(avian)-Like; CRKL rtu elisa kit

• Reactivity: Human
• Sequence Length: 303

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRKL rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRKL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CRKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CRKL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885153
• NCBI GeneID: 1399
• NCBI Accession #: NP_005198.1
• NCBI GenBank Nucleotide #: NM_005207.3
• UniProt Accession #: P46109; A8KA44; D3DX35
• Molecular Weight: 33,777 Da
• NCBI Official Full Name: crk-like protein
• NCBI Official Synonym Full Names: CRK like proto-oncogene, adaptor protein
• NCBI Official Symbol: CRKL
• NCBI Protein Information: crk-like protein
• UniProt Protein Name: Crk-like protein
• Protein Family: Crk-like protein
• UniProt Gene Name: CRKL

NCBI Description

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]

Uniprot Description

May mediate the transduction of intracellular signals.