Human N-Acetyltransferase 1 (NAT1) RTU ELISA Kit | NAT1 rtu elisa kit

Human N-Acetyltransferase 1 (NAT1) ELISA Kit

Cat. Num. AAA4501292

• Gene Names: NAT1; AAC1; MNAT; NATI; NAT-1
• Reactivity: Human
• Synonyms: N-Acetyltransferase 1 (NAT1); Human N-Acetyltransferase 1 (NAT1) ELISA Kit; AAC1; Arylamine N-Acetyltransferase; Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase; NAT1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 290

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.61ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NAT1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NAT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 42741671
• NCBI GeneID: 9
• NCBI Accession #: NP_000653.3
• NCBI GenBank Nucleotide #: NM_000662.7
• UniProt Accession #: P18440; O15159; O15300; Q546N1; Q96TE9; A8K4E7
• Molecular Weight: 33,899 Da
• NCBI Official Full Name: arylamine N-acetyltransferase 1 isoform a
• NCBI Official Synonym Full Names: N-acetyltransferase 1
• NCBI Official Symbol: NAT1
• NCBI Official Synonym Symbols: AAC1; MNAT; NATI; NAT-1
• NCBI Protein Information: arylamine N-acetyltransferase 1
• UniProt Protein Name: Arylamine N-acetyltransferase 1
• Protein Family: Nucleobase-ascorbate transporter
• UniProt Gene Name: NAT1
• UniProt Synonym Gene Names: AAC1; MNAT; NAT-1

NCBI Description

This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens.MiscellaneousNAT1 was historically considered to be monomorphic in nature but reports of allelic variations at the NAT1 locus suggest that it is a polymorphically expressed enzyme.CautionThe allelic variation Ile-149 designated as NAT1*17 is part of the NAT1*11 allelic variation as reported by the nomenclature committee.