Human Growth Differentiation Factor 9 (GDF9) RTU ELISA Kit | GDF9 rtu elisa kit

Human Growth Differentiation Factor 9 (GDF9) ELISA Kit

Cat. Num. AAA459537

• Reactivity: Human
• Synonyms: Growth Differentiation Factor 9 (GDF9); Human Growth Differentiation Factor 9 (GDF9) ELISA Kit; GDF9 rtu elisa kit

• Reactivity: Human
• Sequence Length: 366

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF9 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GDF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GDF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GDF9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 572152931
• NCBI GeneID: 2661
• NCBI Accession #: NP_001275753.1
• NCBI GenBank Nucleotide #: NM_001288824.2
• UniProt Accession #: O60383; Q4VAW5
• Molecular Weight: 51,444 Da
• NCBI Official Full Name: growth/differentiation factor 9 isoform 2
• NCBI Official Synonym Full Names: growth differentiation factor 9
• NCBI Official Symbol: GDF9
• NCBI Protein Information: growth/differentiation factor 9
• UniProt Protein Name: Growth/differentiation factor 9
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF9
• UniProt Synonym Gene Names: GDF-9

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates ovarian function. Reduced expression of this gene may be associated with polycystic ovary syndrome and mutations in this gene may be more common in mothers of dizygotic twins. [provided by RefSeq, Jul 2016]

Uniprot Description

Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.