Porcine Angiopoietin 1 (ANGPT1) ELISA Kit | ANGPT1 elisa kit

Porcine Angiopoietin 1 (ANGPT1) ELISA Kit

Cat. Num. AAA451926

• Gene Names: ANGPT1; AGPT
• Reactivity: Porcine
• Synonyms: Angiopoietin 1 (ANGPT1); Porcine Angiopoietin 1 (ANGPT1) ELISA Kit; ANG1 AGP1 AGPT ANG-I; ANGPT1 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANGPT1. No significant cross-reactivity or interference between ANGPT1 and analogues was observed.
• Sequence Length: 498

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.58ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANGPT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANGPT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPT1 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANGPT1 in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANGPT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ANGPT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ANGPT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ANGPT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47522748
• NCBI GeneID: 397009
• NCBI Accession #: NP_999124.1
• NCBI GenBank Nucleotide #: NM_213959.1
• UniProt Accession #: Q9BDY8
• Molecular Weight: 57,413 Da
• NCBI Official Full Name: angiopoietin-1
• NCBI Official Symbol: ANGPT1
• NCBI Official Synonym Symbols: AGPT
• NCBI Protein Information: angiopoietin-1
• UniProt Protein Name: Angiopoietin-1
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT1
• UniProt Synonym Gene Names: ANG-1

Uniprot Description

Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development ().