Human Glycogen Phosphorylase, Liver (PYGL) RTU ELISA Kit | PYGL rtu elisa kit

Human Glycogen Phosphorylase, Liver (PYGL) ELISA Kit

Cat. Num. AAA4502138

• Gene Names: PYGL; GSD6
• Reactivity: Human
• Synonyms: Glycogen Phosphorylase; Liver (PYGL); Human Glycogen Phosphorylase; Liver (PYGL) ELISA Kit; GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI; PYGL rtu elisa kit

• Reactivity: Human
• Sequence Length: 813

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PYGL rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PYGL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PYGL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PYGL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PYGL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 255653002
• NCBI GeneID: 5836
• NCBI Accession #: NP_001157412.1
• NCBI GenBank Nucleotide #: NM_001163940.1
• UniProt Accession #: P06737; O60567; O60752; O60913; Q501V9; Q641R5; Q96G82; A6NDQ4; B4DUB7; F5H816
• Molecular Weight: 93,134 Da
• NCBI Official Full Name: glycogen phosphorylase, liver form isoform 2
• NCBI Official Synonym Full Names: glycogen phosphorylase L
• NCBI Official Symbol: PYGL
• NCBI Official Synonym Symbols: GSD6
• NCBI Protein Information: glycogen phosphorylase, liver form
• UniProt Protein Name: Glycogen phosphorylase, liver form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGL

NCBI Description

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]

Uniprot Description

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.