Human Glutathione S Transferase Alpha 1 (GSTa1) RTU ELISA Kit | GSTa1 rtu elisa kit

Human Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit

Cat. Num. AAA459742

• Gene Names: GSTA1; GST2; GTH1; GSTA1-1; GST-epsilon
• Reactivity: Human
• Synonyms: Glutathione S Transferase Alpha 1 (GSTa1); Human Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit; GSTA1; GST2; GSTA1-1; GTH1; GST class-alpha member 1; GST HA subunit 1; GST-epsilon; Glutathione S-transferase A1; N-terminally processed; GSTa1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 222

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.265ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSTa1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSTa1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GSTa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSTa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GSTa1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22091454
• NCBI GeneID: 2938
• NCBI Accession #: NP_665683.1
• NCBI GenBank Nucleotide #: NM_145740.4
• UniProt Accession #: P08263; Q14750; Q5GHF8; Q5SZC1
• Molecular Weight: 25,631 Da
• NCBI Official Full Name: glutathione S-transferase A1 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 1
• NCBI Official Symbol: GSTA1
• NCBI Official Synonym Symbols: GST2; GTH1; GSTA1-1; GST-epsilon
• NCBI Protein Information: glutathione S-transferase A1
• UniProt Protein Name: Glutathione S-transferase A1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA1

NCBI Description

This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.