Human Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit | GSTa1 elisa kit

Human Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit

Cat. Num. AAA451624

• Gene Names: GSTA1; GST2; GTH1; GSTA1-1; GST-epsilon
• Reactivity: Human
• Synonyms: Glutathione S Transferase Alpha 1 (GSTa1); Human Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit; GSTA1 GST2 GSTA1-1 GTH1 GST class-alpha member 1 GST HA subunit 1 GST-epsilon Glutathione S-transferase A1; N-terminally processed; GSTa1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GSTa1. No significant cross-reactivity or interference between GSTa1 and analogues was observed.
• Sequence Length: 222

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.265ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GSTa1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GSTa1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSTa1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GSTa1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSTa1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GSTa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSTa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GSTa1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22091454
• NCBI GeneID: 2938
• NCBI Accession #: NP_665683.1
• NCBI GenBank Nucleotide #: NM_145740.4
• UniProt Accession #: P08263; Q14750; Q5GHF8; Q5SZC1
• Molecular Weight: 25,631 Da
• NCBI Official Full Name: glutathione S-transferase A1 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 1
• NCBI Official Symbol: GSTA1
• NCBI Official Synonym Symbols: GST2; GTH1; GSTA1-1; GST-epsilon
• NCBI Protein Information: glutathione S-transferase A1
• UniProt Protein Name: Glutathione S-transferase A1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA1

NCBI Description

This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.