Human Ephrin Type A Receptor 3 (EPHA3) RTU ELISA Kit | EPHA3 rtu elisa kit

Human Ephrin Type A Receptor 3 (EPHA3) ELISA Kit

Cat. Num. AAA459075

• Gene Names: EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
• Reactivity: Human
• Synonyms: Ephrin Type A Receptor 3 (EPHA3); Human Ephrin Type A Receptor 3 (EPHA3) ELISA Kit; ETK; ETK1; HEK; HEK4; TYRO4; Human embryo kinase; Tyrosine-protein kinase receptor ETK1; EPHA3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 983

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.108ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPHA3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EPHA3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EPHA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPHA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EPHA3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21361241
• NCBI GeneID: 2042
• NCBI Accession #: NP_005224.2
• NCBI GenBank Nucleotide #: NM_005233.5
• UniProt Accession #: P29320; Q9H2V3; Q9H2V4
• Molecular Weight: 60,946 Da
• NCBI Official Full Name: ephrin type-A receptor 3 isoform a
• NCBI Official Synonym Full Names: EPH receptor A3
• NCBI Official Symbol: EPHA3
• NCBI Official Synonym Symbols: EK4; ETK; HEK; ETK1; HEK4; TYRO4
• NCBI Protein Information: ephrin type-A receptor 3
• UniProt Protein Name: Ephrin type-A receptor 3
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA3
• UniProt Synonym Gene Names: ETK; ETK1; HEK; TYRO4; EK4; hEK4; Human embryo kinase; Eph-like tyrosine kinase 1

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.