Human Ephrin type-A receptor 3 ELISA Kit | EPHA3 elisa kit

Human Ephrin type-A receptor 3 ELISA Kit

Cat. Num. AAA764325

• Gene Names: EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
• Reactivity: Human
• Synonyms: Ephrin type-A receptor 3; Human Ephrin type-A receptor 3 ELISA Kit; EPHA3/Tyrosine-protein kinase receptor ETK1/Eph-like tyrosine kinase 1/Tyrosine-protein kinase TYRO4/EPH-like kinase 4/EK4/hEK4/HEK/Human embryo kinase/Eph Receptor A3/Cek4/EPH receptor A3/EphA3; EPHA3 elisa kit

• Reactivity: Human
• Sequence Length: 983

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EPHA3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-EPHA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EPHA3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EPHA3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EPHA3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21361241
• NCBI GeneID: 2042
• NCBI Accession #: NP_005224.2
• NCBI GenBank Nucleotide #: NM_005233.5
• Molecular Weight: 60,946 Da
• NCBI Official Full Name: ephrin type-A receptor 3 isoform a
• NCBI Official Synonym Full Names: EPH receptor A3
• NCBI Official Symbol: EPHA3
• NCBI Official Synonym Symbols: EK4; ETK; HEK; ETK1; HEK4; TYRO4
• NCBI Protein Information: ephrin type-A receptor 3
• UniProt Protein Name: Ephrin type-A receptor 3
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA3
• UniProt Synonym Gene Names: ETK; ETK1; HEK; TYRO4; EK4; hEK4; Human embryo kinase; Eph-like tyrosine kinase 1
• UniProt Entry Name: EPHA3_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA3: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family: binds to ephrin-A2, -A3, -A4 and -A5The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Two point mutations seen in a survey of colorectal tumors. Soluble receptors reduce tumor growth and angiogenesis in mouse models. Two alternatively spliced isoforms of EphA3 have been described.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 3p11.2

Cellular Component: early endosome; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; GPI-linked ephrin receptor activity; protein binding

Biological Process: axon guidance; cell adhesion; cell migration; ephrin receptor signaling pathway; peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; regulation of GTPase activity