Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) RTU ELISA Kit | COX4I1 rtu elisa kit

Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit

Cat. Num. AAA458514

• Gene Names: COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
• Reactivity: Human
• Synonyms: Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1); Human Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1; mitochondrial; COX4I1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 169

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COX4I1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COX4I1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COX4I1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COX4I1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COX4I1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 974005293
• NCBI GeneID: 1327
• NCBI Accession #: NP_001305715.1
• NCBI GenBank Nucleotide #: NM_001318786.1
• UniProt Accession #: P13073; Q6P666; B2R4J2; D3DUM7
• Molecular Weight: 19,577 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit 4I1
• NCBI Official Symbol: COX4I1
• NCBI Official Synonym Symbols: COX4; COXIV; COX4-1; COXIV-1; COX IV-1
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• Protein Family: Cytochrome c oxidase
• UniProt Gene Name: COX4I1
• UniProt Synonym Gene Names: COX4; COX IV-1

NCBI Description

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.