Rabbit anti-Human VDAC1 Polyclonal Antibody | anti-VDAC1 antibody

VDAC1, ID (Voltage-dependent Anion-selective Channel Protein 1, VDAC-1, hVDAC1, Outer Mitochondrial Membrane Protein Porin 1, Plasmalemmal Porin, Porin 31HL, Porin 31HM, VDAC)

Cat. Num. AAA629574

• Gene Names: VDAC1; PORIN; VDAC-1; MGC111064
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: VDAC1; Polyclonal Antibody; ID (Voltage-dependent Anion-selective Channel Protein 1; VDAC-1; hVDAC1; Outer Mitochondrial Membrane Protein Porin 1; Plasmalemmal Porin; Porin 31HL; Porin 31HM; VDAC); Anti -VDAC1; anti-VDAC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VDAC1.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-VDAC1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the Center region of human VDAC1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of VDAC1 Antibody (Center) in HL-60, Y79 cell line lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VDAC1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(VDAC1 Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of VDAC1 (arrow) using rabbit polyclonal VDAC1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VDAC1 gene.)

Related Product Information for anti-VDAC1 antibody

VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

Product Categories/Family for anti-VDAC1 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 4507879
• NCBI GeneID: 7416
• NCBI Accession #: NP_003365.1
• NCBI GenBank Nucleotide #: NM_003374.2
• UniProt Accession #: P21796; Q5FVE7; Q9UIQ5; Q9UPL0; B3KVK4; D3DQ93
• Molecular Weight: 30,773 Da
• NCBI Official Full Name: voltage-dependent anion-selective channel protein 1
• NCBI Official Synonym Full Names: voltage-dependent anion channel 1
• NCBI Official Symbol: VDAC1
• NCBI Official Synonym Symbols: PORIN; VDAC-1; MGC111064
• NCBI Protein Information: voltage-dependent anion-selective channel protein 1; porin 31HL; porin 31HM; OTTHUMP00000159381; OTTHUMP00000165946; OTTHUMP00000165947; plasmalemmal porin; outer mitochondrial membrane protein porin 1
• UniProt Protein Name: Voltage-dependent anion-selective channel protein 1
• Protein Family: Voltage-dependent anion-selective channel protein
• UniProt Gene Name: VDAC1
• UniProt Synonym Gene Names: VDAC
• UniProt Entry Name: VDAC1_HUMAN

NCBI Description

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.

Uniprot Description

VDAC1: a protein of the eukaryotic mitochondrial porin family. Forms a voltage-dependent anion channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.

Protein type: Channel, misc.; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: pore complex; mitochondrial outer membrane; membrane; mitochondrion; mitochondrial inner membrane; plasma membrane; nucleus

Molecular Function: protein binding; voltage-gated anion channel activity; porin activity; protein kinase binding

Biological Process: behavioral fear response; viral reproduction; epithelial cell differentiation; apoptosis; nerve-nerve synaptic transmission; learning; transmembrane transport; anion transport