Human Carnitine Palmitoyltransferase 1A, Liver (CPT1A) RTU ELISA Kit | CPT1A rtu elisa kit

Human Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit

Cat. Num. AAA458542

• Gene Names: CPT1A; CPT1; CPT1-L; L-CPT1
• Reactivity: Human
• Synonyms: Carnitine Palmitoyltransferase 1A; Liver (CPT1A); Human Carnitine Palmitoyltransferase 1A; Liver (CPT1A) ELISA Kit; CPT1-L; Carnitine O-palmitoyltransferase I; liver isoform; CPT1A rtu elisa kit

• Reactivity: Human
• Sequence Length: 756

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.29ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPT1A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPT1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CPT1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CPT1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CPT1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 73623028
• NCBI GeneID: 1374
• NCBI Accession #: NP_001027017.1
• NCBI GenBank Nucleotide #: NM_001031847.2
• UniProt Accession #: P50416; Q8TCU0; Q9BWK0
• Molecular Weight: 86,239 Da
• NCBI Official Full Name: carnitine O-palmitoyltransferase 1, liver isoform isoform 2
• NCBI Official Synonym Full Names: carnitine palmitoyltransferase 1A
• NCBI Official Symbol: CPT1A
• NCBI Official Synonym Symbols: CPT1; CPT1-L; L-CPT1
• NCBI Protein Information: carnitine O-palmitoyltransferase 1, liver isoform
• UniProt Protein Name: Carnitine O-palmitoyltransferase 1, liver isoform
• Protein Family: Carnitine O-palmitoyltransferase
• UniProt Gene Name: CPT1A
• UniProt Synonym Gene Names: CPT1; CPT1-L; CPT I; CPTI-L

NCBI Description

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism.