Rabbit anti-Human DUSP3 Polyclonal Antibody | anti-DUSP3 antibody

DUSP3, CT (Dual Specificity Protein Phosphatase 3, Dual Specificity Protein Phosphatase VHR, VHR) (PE)

Cat. Num. AAA6473121

• Gene Names: DUSP3; VHR
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: DUSP3; Polyclonal Antibody; CT (Dual Specificity Protein Phosphatase 3; Dual Specificity Protein Phosphatase VHR; VHR) (PE); anti-DUSP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DUSP3.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DUSP3 antibody: FLISA, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 157-185 in the C-terminal region of human DUSP3, UniProt Accession #NP_004081; P51452.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2 ug/lane) using MBS631177 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP3 gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS631177 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS631177 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS631177 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DUSP3 antibody

DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. The gene for this protein maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer.

Product Categories/Family for anti-DUSP3 antibody

Antibodies; Enzymes; Phosphatase

References

1. Alonso, A., et al., Nat. Immunol. 4(1):44-48 (2003). 2. Alonso, A., et al., J. Biol. Chem. 276(7):4766-4771 (2001). 3. Todd, J.L., et al., J. Biol. Chem. 274(19):13271-13280 (1999). 4. Kamb, A., et al., Genomics 23(1):163-167 (1994). 5. Folander, K., et al., Genomics 23(1):295-296 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 4758208
• NCBI GeneID: 1845
• NCBI Accession #: NP_004081
• NCBI GenBank Nucleotide #: NM_004090.3
• UniProt Accession #: P51452; Q5U0J1; Q8IYJ9; D3DX45
• Molecular Weight: 16,163 Da
• NCBI Official Full Name: dual specificity protein phosphatase 3
• NCBI Official Synonym Full Names: dual specificity phosphatase 3
• NCBI Official Symbol: DUSP3
• NCBI Official Synonym Symbols: VHR
• NCBI Protein Information: dual specificity protein phosphatase 3
• UniProt Protein Name: Dual specificity protein phosphatase 3
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP3
• UniProt Synonym Gene Names: VHR; VHR
• UniProt Entry Name: DUS3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; cytoplasm; immunological synapse; cytosol; nucleus

Molecular Function: MAP kinase phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: negative regulation of JNK cascade; nerve growth factor receptor signaling pathway; in utero embryonic development; negative regulation of MAPKKK cascade; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; negative regulation of T cell receptor signaling pathway; toll-like receptor 3 signaling pathway; positive regulation of mitotic cell cycle; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; negative regulation of T cell activation; inactivation of MAPK activity